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首页> 外文期刊>The Annals of otology, rhinology, and laryngology >Bioluminescent imaging of pneumococcal otitis media in chinchillas
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Bioluminescent imaging of pneumococcal otitis media in chinchillas

机译：龙猫肺炎球菌中耳炎的生物发光成像

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Objectives: Bioluminescent imaging has emerged as a powerful tool for monitoring the pathological process of infections in animals. The purpose of this study was to harness this new tool for objective assessment of acute otitis media (AOM) in animals with and without antibiotic interventions. Methods: Thirty-six healthy chinchillas, free of middle ear infections, were randomly divided into a control group and a group that received amoxicillin treatment. Bioluminescent Streptococcus pneumoniae (Xen 10) was injected into the epitympanic bullae of chinchillas (50 colony-forming units each) for induction of AOM. The infectious process of Xen 10 in the bullae of living animals with and without antibiotic interventions was monitored in real time with bioluminescence equipment. Results: A dynamic change of bioluminescent signals in the bullae of chinchillas from days 1 to 14 was observed after Xen 10 injection. Amoxicillin treatment reduced the bioluminescent signals in the bullae of chinchillas compared with controls. The AOM persisted for 14 days, and middle ear effusion for 6 weeks, in the control animals, whereas AOM lasted for 2 days, and effusion for 6 to 12 days, in the antibiotic-treated animals. Conclusions: Bioluminescent imaging provides an innovative method for assessment of the bacterial loads in the middle ear of chinchillas in a real-time manner and is very useful for objective evaluation of the efficacy of therapeutic interventions.
机译：目标：生物发光成像已成为监测动物感染病理过程的强大工具。这项研究的目的是利用这种新工具客观评估动物是否接受抗生素干预后的急性中耳炎（AOM）。方法：将36只健康的龙猫无中耳感染，随机分为对照组和接受阿莫西林治疗的组。将生物发光性肺炎链球菌（Xen 10）注射入龙猫的鼓室大疱（各50个菌落形成单位），以诱导AOM。使用生物发光设备实时监测Xen 10在有或没有抗生素干预下的活动物大疱中的感染过程。结果：Xen 10注射后，从第1天到第14天，在龙猫的大疱中观察到生物发光信号的动态变化。与对照相比，阿莫西林处理减少了龙猫大疱中的生物发光信号。在对照动物中，AOM持续14天，中耳积液持续6周，而在用抗生素治疗的动物中，AOM持续2天，积液持续6至12天。结论：生物发光成像提供了一种实时评估龙猫中耳细菌负荷的创新方法，对于客观评估治疗干预的有效性非常有用。

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