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首页> 外文期刊>Chromatographia >Simultaneous determination of nalbuphine and opiates in human hair by gas chromatography-mass spectrometry
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Simultaneous determination of nalbuphine and opiates in human hair by gas chromatography-mass spectrometry

机译：气相色谱-质谱法同时测定人发中的纳布啡和鸦片剂

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摘要

The method for simultaneous determination of nalbuphine and opiates (codeine, morphine, and 6-monoocetylmorpkine) in human hair was developed in the selected-ion monitoring (SIM) mode of a gas chromatography-mass spectrometer (GC-MS). Thirty-milligram hair samples were incubated in 0.01 M HCl overnight at 50 degreesC. We extracted the drugs from resulting hydrolyzed solutions with a mixture of chloroform-isopropanol-n-keptone (50:17:33, v/v/v) at pH 9.2. The residues were then evaporated, derivatized, and injected into the GC-MS with the internal standards. The limits of quantification for codeine, morphine, 6-monoacet morphine, and nalbuphine were 0.26, 0.21, 0.24, and 0.18 ng mg(-1), respectively. Drug recoveries fell in the range 72.4-86.5%. The responses were linear, with correlation coefficients (r > 0.9958) for the drugs studied. This study also demonstrated that hair dyeing or bleaching diminished the concentration level of nallouphine, in vitro.
机译：以气相色谱-质谱仪（GC-MS）的选择离子监测（SIM）模式开发了一种同时测定人发中的纳布啡和鸦片剂（可待因，吗啡和6-单乙酰基吗啡）的方法。将三十毫克的头发样品在0.01 M HCl中于50℃孵育过夜。我们从pH 9.2的氯仿-异丙醇-正庚酮的混合物（50：17：33，v / v / v）中，从所得的水解溶液中提取药物。然后将残留物蒸发，衍生化并与内标一起注入GC-MS中。可待因，吗啡，6-单乙酰吗啡和纳布啡的定量限分别为0.26、0.21、0.24和0.18 ng mg（-1）。药物回收率在72.4-86.5％之间。响应呈线性关系，所研究药物的相关系数（r> 0.9958）。这项研究还表明，在体外染发或漂白可降低纳洛啡的浓度水平。

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