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Liposome capillary electrophoresis of peptides and proteins

机译：肽和蛋白质的脂质体毛细管电泳

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Liposome capillary electrophoresis (LCE) using unilamellar liposomes composed of the zwitterionic phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) as a suspended pseudo stationary phase has been investigated for its capability at separating peptides and proteins in bare fused-silica capillaries. The study has explored different strategies for allowing the liposome suspension to act as a disperse pseudo stationary phase with the ability of modulating selectivity, resolution and separation performance of peptides and proteins in bare-fused silica capillaries. Suck strategies comprise the use of capillaries either partially or totally filled with the liposome suspension, whereas the electrolyte solution is liposome-free, or the incorporation of the liposomes into the buffer solution employed for rinsing the capillary and as the background electrolyte. Three synthetic peptides of similar amino acid sequence and four basic standard proteins have been employed as test analytes. Varying the volume of the liposome suspension introduced in the capillary promoted differentiated variations in the migration velocity of the three peptides reflecting their selective interactions with the liposomes. Efficient separation of basic proteins was obtained at pH 7.4 in a bare fused-silica capillary with the electrolyte solution containing 60 muM POPC.
机译：已经研究了脂质体毛细管电泳（LCE），该脂质体使用由两性离子磷脂1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）组成的单层脂质体作为悬浮的伪固定相，具有分离肽和蛋白质的能力。裸露的熔融石英毛细管。这项研究探索了各种不同的策略，以使脂质体悬浮液充当分散的假固定相，并具有调节裸露的熔融石英毛细管中肽和蛋白质的选择性，分辨率和分离性能的能力。吸取策略包括使用部分或全部充满脂质体悬浮液的毛细管，而电解质溶液不含脂质体，或将脂质体掺入用于冲洗毛细管和作为背景电解质的缓冲溶液中。三种具有相似氨基酸序列的合成肽和四种基本标准蛋白质已被用作测试分析物。引入毛细管中的脂质体悬浮液的体积变化促进了三种肽迁移速度的差异化变化，反映了它们与脂质体的选择性相互作用。在裸露的熔融石英毛细管中，使用含有60μMPOPC的电解质溶液，可以在pH 7.4的条件下有效分离碱性蛋白质。

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