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Avidin and streptavidin in clinical diagnostics

机译:抗生物素蛋白和链霉亲和素在临床诊断中的作用

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摘要

Still in the early 1980s diagnostic tests were using directly coated tubes, particles, or microtiter plates as solid phases. At that time coating was examined more qualitatively, incubation time was long (several hours), and sensitivity was generally lower than today. In the mid 1980s SA~1 entered the clinical lab to replace directly bound antibodies and antigens as matrices. In the hematology labs FACS equipment (fluorescence activated cell sorter) became standard, and leucocyte typing workshops established the cellular markers. The technology in the field of imaging improved slowly but steadily.
机译:仍然在1980年代初期,诊断测试仍使用直接涂覆的试管,颗粒或微量滴定板作为固相。那时,对涂层进行了更定性的检查,孵育时间长(几个小时),灵敏度通常比今天低。在1980年代中期,SA〜1进入临床实验室,以取代直接结合的抗体和抗原作为基质。在血液学实验室中,FACS设备(荧光激活细胞分选仪)成为标准设备,白细胞分型车间建立了细胞标记物。成像领域的技术进步缓慢但稳定。

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