Applications of a peptide ligand for streptavidin: the Strep-tag

Arne Skerra; Thomas G.M. Schmidt

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Applications of a peptide ligand for streptavidin: the Strep-tag

机译：链霉亲和素的肽配体的应用：链球菌标签

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The strep-tag constitutes a nine amino acid-peptide that binds specifically to streptavidin and occupies the same pocket where biotin is normally complexed. Since the Strep-tag participates in a reversible interaction it can be applied for the efficient purification of corresponding fusion proteins on affinity columns with immobilized streptavidin. Elution of the bound recombinant protein can be effected under mild buffer conditions by competition with biotin or a suitable derivative. In addition, Strep-tag fusion proteins can be easily detected in immunochemical assays, like Western blots or ELISAs, by means of commercially available streptavidin-enzyme conjugates. The Strep-tag /streptavidin system has been systematically optimized over the past years, including the engineering of streptavidin itself. Structural insight into the molecular mimicry between the peptide and biotin was furthermore gained from X-ray crystallographic analysis. As a result the system provides a reliable and versatile tool in recombinant protein chemistry. Exemplary applications of the Strep-tag are discussed in this review.

机译：所述strep标签构成了九个氨基酸的肽，其特异性地与链霉亲和素结合并且占据了通常复合生物素的相同口袋。由于Strep-tag参与可逆相互作用，因此可用于固定化链霉亲和素在亲和柱上有效纯化相应的融合蛋白。结合的重组蛋白的洗脱可以在温和的缓冲液条件下通过与生物素或合适的衍生物竞争来实现。此外，借助于市售的抗生蛋白链菌素-酶结合物，可以在免疫化学测定（如Western印迹或ELISA）中轻松检测到Strep-tag融合蛋白。在过去的几年中，已对Strep-tag / streptavidin系统进行了系统优化，包括streptavidin本身的工程设计。通过X射线晶体学分析，进一步了解了肽和生物素之间的分子模拟结构。结果，该系统为重组蛋白化学提供了可靠且通用的工具。在这篇综述中讨论了Strep-tag的示例性应用。

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《Biomolecular engineering》 |1999年第4期|共8页
作者
Arne Skerra; Thomas G.M. Schmidt;
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正文语种 eng
中图分类遗传学;医用一般科学;
关键词
protein expression; affinity tag; purification; detection;

机译：蛋白表达;亲和标签;纯化;检测;

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专利

1. Applications of a peptide ligand for streptavidin: the Strep-tag [J] . Arne Skerra, Thomas G.M. Schmidt Biomolecular engineering . 1999,第1a4期

机译：链霉亲和素的肽配体的应用：链球菌标签
2. Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. [J] . Korndorfer IP, Skerra A Protein Science: A Publication of the Protein Society . 2002,第4期

机译：工程链霉抗生物素蛋白对链球菌标签II肽的亲和力提高是由于在结合位点上的盖状环具有固定的开放构象。
3. MUTAGENESIS OF A FLEXIBLE LOOP IN STREPTAVIDIN LEADS TO HIGHER AFFINITY FOR THE STREP-TAG II PEPTIDE AND IMPROVED PERFORMANCE IN RECOMBINANT PROTEIN PURIFICATION [J] . Voss S., Skerra A. Protein Engineering . 1997,第8期

机译：链霉抗生物素蛋白中的柔性环诱变以提高STREP-TAG II肽的亲和力，并提高重组蛋白纯化的性能
4. Synthesis and Analysis of Peptide Ligand for Biosensor Application Using Combinatorial Chemistry: Screening and Characterization of 2,3,7-Trichlorodibenzo-p-dioxin-Binding Peptide [C] . Yasutaka Morita, Yuji Murakami, Kenji Yokoyama, PacifiChem 2000 Conference Dec, 2000 Honolulu, Hawaii . 2000

机译：组合化学的生物传感器应用肽配体的合成和分析：2,3,7-三氯二苯并-p-二恶英结合肽的筛选和表征
5. Investigating cell adhesion to controlled surface chemistry via self-assembly of binary composition alkylthiol monolayers, streptavidin immobilization, and cell receptor ligand attachment. [D] . Nelson, Kjell Erik. 2003

机译：通过二元组合烷硫醇单分子层的自组装，链霉亲和素固定和细胞受体配体附着，研究细胞对受控表面化学的粘附。
6. Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site [O] . Ingo P. Korndörfer, Arne Skerra 2002

机译：工程链霉亲和素对链球菌标签II肽的亲和力提高是由于在结合位点上盖状环的固定开放构象
7. Non-covalent ligand conjugation to biotinylated DNA nanoparticles using TAT peptide genetically fused to monovalent streptavidin [O] . Wenchao Sun, David Fletcher, Rolf Christiaan van Heeckeren, 2012

机译：使用Tat肽的生物素化DNA纳米颗粒的非共价配体缀合物遗传融合给单价链霉抗生物素蛋白

Applications of a peptide ligand for streptavidin: the Strep-tag

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