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Direct force measurements of the streptavidin-biotin interaction

机译：链亲和素-生物素相互作用的直接力测量

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摘要

The interaction between streptavidin and its ligand, biotin, were studied by direct force measurements. The complimentary approaches of surface force apparatus (SFA) and atomic force microscopy (AFM) were used to elucidate both long-range and short-range adhesive interactions of the streptavidin-biotin interaction. The high spatial resolution of the SFA provided a detailed profile of the intersurface forces of apposing surfaces functionalized with streptavidin and biotin. Measurements obtained by the SFA corresponded to long and intermediate-range forces that are important in determining ligand-receptor association. AFM was used to measure the unbinding force of individual streptavidin-biotin complexes. These measurements revealed the short-range interactions (i.e. hydrophobic and hydrogen bonding forces) that stabilize the intermolecular bond.
机译：通过直接力测量研究了链霉亲和素与其配体生物素之间的相互作用。使用表面力仪器（SFA）和原子力显微镜（AFM）的互补方法来阐明链霉亲和素-生物素相互作用的长距离和短距离粘合相互作用。 SFA的高空间分辨率提供了用链霉亲和素和生物素功能化的对置表面的表面间力的详细资料。通过SFA获得的测量值对应于在确定配体-受体缔合中很重要的长程和中程力。原子力显微镜用于测量单个链霉亲和素-生物素复合物的解结合力。这些测量揭示了稳定分子间键的短程相互作用（即疏水和氢键力）。

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