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首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Mixed Fluorocarbon/Hydrocarbon Surfactant Vesicles as Carriers of Metalloproteins: Scattering and Magnetic Resonance Experiments
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Mixed Fluorocarbon/Hydrocarbon Surfactant Vesicles as Carriers of Metalloproteins: Scattering and Magnetic Resonance Experiments

机译：碳氟化合物和碳氢化合物混合表面活性剂囊泡作为金属蛋白的载体：散射和磁共振实验

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摘要

Spontaneously formed mixed vesicles were prepared with n-dodecylbetaine and the ammonium salt of perfluoropolyether carboxylate, and they were loaded with the water-soluble beef heart cytochrome c. The resulting dispersions were characterized with light and neutron scattering patterns, UV-vis spectra, and magnetic resonances (NMR and ESR). Proteins-containing vesicles showed scattering patterns that were interpreted in terms of a decrease of the membrane curvature with a resultant increase of the vesicle size that ranged from 83 to 113 nm, depending on the cytochrome c concentration in the dispersion. The interactions between surfaces of protein and of vesicle membrane, which could be responsible for the decreased curvature, did not alter either the optical properties of the heme unit of the protein (from UV-vis) or the dynamics of the vesicle membrane (from ~1H NMR of betaine and ESR of membrane-soluble doxylstearic acids). Cytochrome c was therefore suggested to be surrounded by a shell of water according to the so-called water-shell model. A comparison was made with the behavior of hydrophobic porphyrins introduced in the same vesicle system, as previously reported from the authors.
机译：用正十二烷基甜菜碱和全氟聚醚羧酸盐的铵盐制备自发形成的混合囊泡，并在其中装载水溶性牛肉心细胞色素c。所得的分散体通过光和中子散射图，紫外可见光谱和磁共振（NMR和ESR）进行表征。含蛋白质的囊泡表现出散射模式，这取决于膜曲率的减小，其结果是囊泡大小的增加范围为83至113 nm，具体取决于分散液中细胞色素c的浓度。蛋白质和囊泡膜表面之间的相互作用可能导致曲率降低，但不会改变蛋白质血红素单元的光学性质（来自UV-vis），也不会改变囊泡膜的动力学（来自〜甜菜碱的1 H NMR和膜溶性二硬脂酸的ESR）。因此，根据所谓的水壳模型，建议细胞色素c被水壳包围。如作者先前报道，将疏水性卟啉引入同一囊泡系统的行为进行了比较。

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