Two-dimensional crystallization of streptavidin: in pursuit of the molecular origins of structure, morphology, and thermodynamics

William R. Schief; Todd Edwards; Wolfgang Frey

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Two-dimensional crystallization of streptavidin: in pursuit of the molecular origins of structure, morphology, and thermodynamics

机译：链霉亲和素的二维结晶：追求结构，形态和热力学的分子起源

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The streptavidin two-dimensional (2D) crystallization model has served as a paradigm for molecular self-assembly at interfaces. We have developed quantitative Brewster angle microscopy for the in situ measurement of spatially resolved relative protein: surface densities. This allows investigation of both the thermodynamics and morphologies of 2D crystal growth. For crystal structure analysis, we employ TEM on grown crystals transferred to solid substrates. Comparison of results between commercially available streptavidin, recombinant streptavidin, and site-directed streptavidin mutants has provided insight into the proteinprotein and protein-lipid interactions that underlie 2D crystallization.

机译：链霉亲和素二维（2D）结晶模型已成为界面分子自组装的范例。我们开发了定量布鲁斯特角显微镜，用于原位测量空间分辨的相对蛋白：表面密度。这允许研究2D晶体生长的热力学和形态。对于晶体结构分析，我们对转移到固体基质上的生长晶体采用TEM。对市售链霉亲和素，重组链霉亲和素和定点链霉亲和素突变体之间的结果进行比较，可以深入了解构成2D结晶的蛋白质和蛋白质-脂质相互作用。

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《Biomolecular engineering》 |1999年第4期|共10页
作者
William R. Schief; Todd Edwards; Wolfgang Frey;
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正文语种 eng
中图分类遗传学;医用一般科学;
关键词
BAM; interfacial dynamics; two-dimensional crystals; streptavidin;

机译：BAM;界面动力学;二维晶体;链霉亲和素;

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1. Two-dimensional crystallization of streptavidin: in pursuit of the molecular origins of structure, morphology, and thermodynamics [J] . William R. Schief, Todd Edwards, Wolfgang Frey Biomolecular engineering . 1999,第1a4期

机译：链霉亲和素的二维结晶：追求结构，形态和热力学的分子起源
2. Relationships between molecular structure and kinetic and thermodynamic controls in lipid systems: Part III. Crystallization and phase behavior of 1-palmitoyl-2,3-stearoyl-sn-glycerol (PSS) and tristearoylglycerol (SSS) binary system [J] . Bouzidi L., Narine S.S. Chemistry and Physics of Lipids . 2012,第1期

机译：脂质系统中分子结构与动力学和热力学控制之间的关系：第三部分。 1-棕榈酰-2,3-硬脂酰-sn-甘油（PSS）和三硬脂酰甘油（SSS）二元体系的结晶和相行为
3. Solid-Liquid Interfaces: Molecular Structure, Thermodynamics, and Crystallization [J] . Mark Asta, Frans Spaepen, J. Friso van der Veen, MRS bulletin . 2004,第12期

机译：固液界面：分子结构，热力学和结晶
4. Effect of Crystallization Temperature on the Morphology of High Pressure Crystallized Ultra-high Molecular Weight Polyethylene [C] . Mary E.Turell, Anuj Bellare PMSE Symposia . 2002

机译：结晶温度对高压结晶超高分子量聚乙烯形态的影响
5. Investigating streptavidin crystallization on lipid monolayers and bilayer vesicles. [D] . Ratanabanangkoon, Pasut. 2002

机译：研究链霉亲和素在脂质单层和双层囊泡上的结晶。
6. Two-dimensional crystallization of streptavidin by nonspecific binding to a surface film: study with a scanning electron microscope. [O] . T Furuno, H Sasabe 1993

机译：非特异性结合至表面膜的链霉亲和素的二维结晶：用扫描电子显微镜研究。
7. Two-dimensional crystallization of streptavidin by nonspecific binding to a surface film: study with a scanning electron microscope. [O] . Furuno, T, Sasabe, H 1993

机译：非特异性结合至表面膜的链霉亲和素的二维结晶：用扫描电子显微镜研究。
8. Material and Structures. Molecular Structure and Thermodynamics Program. Part I, Molecular Structure [R] . Siegel, S., Eisenthal, K. B. 1962

机译：材料和结构。分子结构和热力学程序。第一部分，分子结构

Two-dimensional crystallization of streptavidin: in pursuit of the molecular origins of structure, morphology, and thermodynamics

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