...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomolecular engineering >An evaluation of the use of two-dimensional gel electrophoresis in proteomics
【24h】

An evaluation of the use of two-dimensional gel electrophoresis in proteomics

机译：蛋白质组学中二维凝胶电泳的使用评估

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

With whole genomes being sequenced almost routinely, the next logical step towards a better understanding of cellular mechanisms lies in studying the functional units of gene expression-proteins. One of the fundamental approaches in proteomics is the use of two-dimensional gel electrophoresis as a mode of separation and visualization of complex protein mixtures. Despite several limitations of the method, its ability to separate large numbers of proteins, including their post-translationally modified forms, ensures that it will continue to be popular in several well-defined areas of proteomics. In this article, we discuss the merits and drawbacks of two-dimensional gels and compare them with alternative systems such as one-dimensional gels and liquid chromatography-based separation methods. In the wake of recent advances in mass spectrometry and related areas, we outline areas where two-dimensional gels can best be utilized as the preferred separation method in proteomic strategies.
机译：由于几乎可以对整个基因组进行常规测序，因此更好地了解细胞机制的下一个逻辑步骤是研究基因表达蛋白的功能单元。蛋白质组学的基本方法之一是使用二维凝胶电泳作为复杂蛋白质混合物的分离和可视化方式。尽管该方法有一些局限性，但其分离大量蛋白质（包括其翻译后修饰形式）的能力确保了它在蛋白质组学的几个明确定义的领域继续受欢迎。在本文中，我们将讨论二维凝胶的优缺点，并将它们与一维凝胶和基于液相色谱的分离方法等替代系统进行比较。随着质谱技术和相关领域的最新发展，我们概述了二维凝胶最适合用作蛋白质组学策略中优选分离方法的领域。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号