Block-replacement mutagenesis for functional dissection of multiple transcription factor complexes

Milota Kaluzova; Stefan Kaluz

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Block-replacement mutagenesis for functional dissection of multiple transcription factor complexes

机译：块替代诱变用于多种转录因子复合物的功能解剖

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摘要

We propose a simple method for investigation of roles of individual transcription factors in complexes of multiple factors bound to the same cis element. By block-replacement mutagenesis, the whole cis element is replaced with a new one containing a binding site for a single factor. From the reporter activity of the mutant promoter construct, the importance of the individual factor in transciptional activation is deduced. This approach allowed us to functionally identify SP1 as the most important PR1 binding transcription factor in the MN promoter.

机译：我们提出了一种简单的方法来研究单个转录因子在与同一顺式元件结合的多个因子复合物中的作用。通过块置换诱变，整个顺式元件被包含一个单一因子结合位点的新元件取代。从突变启动子构建体的报道分子活性中，可以推断出个体因子在转录激活中的重要性。这种方法使我们能够在功能上将SP1鉴定为MN启动子中最重要的PR1结合转录因子。

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《Biomolecular engineering》 |2001年第1期|共3页
作者
Milota Kaluzova; Stefan Kaluz;
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正文语种 eng
中图分类遗传学;医用一般科学;
关键词
block-replacement mutagenesis; transcription factors; cis element;

机译：块置换诱变转录因子顺式元件;

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专利

1. Block-replacement mutagenesis for functional dissection of multiple transcription factor complexes [J] . Milota Kaluzova, Stefan Kaluz Biomolecular engineering . 2001,第1期

机译：块替代诱变用于多种转录因子复合物的功能解剖
2. Functional dissection of the B" component of RNA polymerase III transcription factor IIIB: a scaffolding protein with multiple roles in assembly and initiation of transcription. [J] . A Kumar, G A Kassavetis, E P Geiduschek, Molecular and Cellular Biology . 1997,第4期

机译：RNA聚合酶III转录因子IIIB的B“组分的功能解剖：一种在组装和转录起始中具有多种作用的支架蛋白。
3. Functional dissections between GAMYB and dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone [J] . Washio K Plant physiology . 2003,第2期

机译：GAMYB和自由度转录因子之间的功能解剖表明蛋白质-蛋白质关联在赤霉素介导的水稻糊粉中RAmy1A基因表达中的作用
4. Transcriptional Profiling and Functional Genomics Reveal a Role for AHR Transcription Factor in Nephrogenesis [C] . KENNETH S. RAMOS Conference on "Framing the Future in Light of the Past: Living in a Chemical World" . 2006

机译：转录分析和功能基因组学揭示了肾病AHR转录因子的作用
5. Novel optical techniques for exploring the DNA binding affinity and specificity of eukaryotic transcription factors and transcription factor complexes. [D] . Bonham, Andrew James. 2010

机译：探索真核转录因子和转录因子复合物的DNA结合亲和力和特异性的新型光学技术。
6. Functional dissection of the B component of RNA polymerase III transcription factor IIIB: a scaffolding protein with multiple roles in assembly and initiation of transcription. [O] . A Kumar, G A Kassavetis, E P Geiduschek, 1997

机译：RNA聚合酶III转录因子IIIB的B组分的功能解剖：一种在组装和转录起始中具有多种作用的支架蛋白。
7. Functional dissection of the B" component of RNA polymerase III transcription factor IIIB: a scaffolding protein with multiple roles in assembly and initiation of transcription. [O] . Kumar, A, Kassavetis, G A, Geiduschek, E P, 1997

机译：RNA聚合酶III转录因子IIIB的B“组分的功能解剖：一种在组装和转录起始中具有多种作用的支架蛋白。

Block-replacement mutagenesis for functional dissection of multiple transcription factor complexes

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