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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Design of a Membrane Fluorescent Sensor Based on Photo-Cross-Linked PEG Hydrogel
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Design of a Membrane Fluorescent Sensor Based on Photo-Cross-Linked PEG Hydrogel

机译：基于光交联PEG水凝胶的膜荧光传感器设计

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摘要

A polymeric hydrogel film was made photochemically for the fluorescent detection of copper ions. An eight-branched PEG macromer having a nitrocinnamate (NC) moiety as the pendant group was synthesized and found to form a gel upon exposure to 365-nm irradiation in the absence of any photoinitiators or catalysts. Peptide 1 (Dns-Gly-His-Lys(NC)) was synthesized with Fmoc solid-phase method and contained three essential parts: a dansyl fluorophore for fluorescent signal transduction, a metal binding site for selective recognition of copper ions from aqueous solution, and a NC group for interaction with PEG-NC macromer. The peptide was found to be efficiently immobilized within the hydrogel network during the photo-cross-linking process. The prepared PEG-NC/1 gel film is strongly fluorescent and can be quenched by the presence of copper ions. The quenching process was reversed by acid treatment and an excellent reversibility was obtained. The designed membrane sensor showed a superior selectivity toward copper ions as demonstrated by fluorescence spectroscopy and epifluorescence imaging.
机译：用光化学方法制备聚合物水凝胶薄膜，用于荧光检测铜离子。合成了具有硝基肉桂酸酯（NC）部分作为侧基的八支PEG大分子单体，发现在没有任何光引发剂或催化剂的情况下暴露于365 nm辐射后形成凝胶。肽1（Dns-Gly-His-Lys（NC））是采用Fmoc固相方法合成的，包含三个基本部分：用于荧光信号转导的丹磺酰荧光团，用于选择性识别水溶液中铜离子的金属结合位点，和一个与PEG-NC大分子单体相互作用的NC基团。发现该肽在光交联过程中被有效地固定在水凝胶网络内。制备的PEG-NC / 1凝胶膜具有强荧光性，可以通过铜离子的存在而淬灭。通过酸处理使淬火过程逆转，获得了优异的可逆性。如荧光光谱和落射荧光成像所证明，设计的膜传感器对铜离子显示出优异的选择性。

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