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首页> 外文期刊>Biomolecular engineering >In vitro studies of human and rat osteoclast activity on hydroxyapatite, β-tricalcium phosphate, calcium carbonate
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In vitro studies of human and rat osteoclast activity on hydroxyapatite, β-tricalcium phosphate, calcium carbonate

机译：人和大鼠破骨细胞对羟磷灰石，β-磷酸三钙，碳酸钙的体外研究

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Investigations on the ceramic degradation caused by osteoclasts are designed to asses osteoclast-ceramic interactions and to determine which ceramics are more suitable for use as bone substitute. This study investigated the resorptive activity of osteoclasts on ceramics presenting different solubility rates. Osteoclasts isolated from new-born rat and from human giant cell tumour were cultured on different bioceramics: hydroxyapatite (HA), β-tricalcium phosphate (TCP) and calcium carbonate (calcite). Cytoskeletal was revealed by actin labelling and ceramic surfaces were observed by scanning electron microscopy (SEM). On all materials, the distribution of actin in typical ring was revealed. SEM examinations showed a clear difference in the shape and the depth of resorption lacunae on different ceramics. On pure HA, a superficial attack, clearly visible but very little extended. Numerous resorption lacunae, deep and well-delimited were observed on pure β-TCP, but attacks less punctually were detected too. On pure calcite, an attack with form of spikes, very widespread but superficial were revealed. Degradation measurements revealed a significant increase of P release from the phosphocalcic ceramics and of Ca from all ceramics in the presence of osteoclasts. The both cell models found these characteristics, the rat osteoclasts were also an excellent model to study the ceramic resorption.
机译：对破骨细胞引起的陶瓷降解的研究旨在评估破骨细胞与陶瓷的相互作用，并确定哪种陶瓷更适合用作骨替代物。这项研究调查了破骨细胞对呈现不同溶解度的陶瓷的吸收活性。从新生大鼠和人类巨细胞瘤中分离出的破骨细胞在不同的生物陶瓷上培养：羟基磷灰石（HA），β-磷酸三钙（TCP）和碳酸钙（方解石）。通过肌动蛋白标记揭示细胞骨架，并通过扫描电子显微镜（SEM）观察陶瓷表面。在所有材料上，揭示了肌动蛋白在典型环中的分布。 SEM检查表明，在不同陶瓷上的形状和深度都存在明显的差异。在纯HA上，是一种表面攻击，清晰可见，但几乎没有扩展。在纯β-TCP上观察到了许多深层和界限分明的吸收腔，但也发现了较少的准时攻击。在纯方解石上，发现了一种尖峰形式的攻击，这种攻击非常普遍，但表面很浅。降解测量表明，在存在破骨细胞的情况下，磷钙陶瓷中P的释放量显着增加，所有陶瓷中Ca的释放量显着增加。两种细胞模型都发现了这些特性，大鼠破骨细胞也是研究陶瓷吸收的优秀模型。

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