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Cell-extracellular matrix interaction and physico-chemical characteristics of titanium surfaces depend on the roughness of the material

机译：钛表面的细胞-细胞外基质相互作用和理化特性取决于材料的粗糙度

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The interaction of cells with the extracellular matrix at the interface of an implant determines the biology of cells and tissues. We analysed components of cell adhesion and measured physico-chemical characteristics of structural modifications of titanium surfaces: polished, machined, glass particle-blasted, corundum-blasted, vacuum plasma-sprayed. Scanning electron microscopy and profilometry revealed a differentiated topography from smooth to rough surfaces, respectively. Osteoblastic MG-63 cells showed an increased spreading on surfaces with low roughness, although without a straight correlation with the surface topography. Integrin expression was increased on structured surfaces compared with polished material, and the organization of the actin cytoskeleton and fibronectin was impaired on extremely rough surfaces. Electrochemical methods, especially the electrochemical impedance spectroscopy (EIS) was used to evaluate physico-chemical characteristics, and the impedance curves revealed a dependence on the roughness of the material surfaces. Further analyses of the EIS results were performed using equivalent circuits which model the electrical flow through the interface. First indications for a correlation between parameters from the equivalent circuits with surface properties were obtained which promise a relevance for the biological response of the cells.
机译：细胞与植入物界面处的细胞外基质的相互作用决定了细胞和组织的生物学特性。我们分析了细胞粘附的成分，并测量了钛表面结构修饰的物理化学特征：抛光，机械加工，玻璃微粒喷砂，刚玉喷砂，真空等离子喷涂。扫描电子显微镜和轮廓测定法分别显示了从光滑表面到粗糙表面的不同形貌。破骨细胞MG-63细胞在低粗糙度的表面上显示出增加的扩散，尽管与表面形貌没有直接的关系。与抛光材料相比，整合素在结构化表面上的表达增加，并且在极粗糙的表面上肌动蛋白细胞骨架和纤连蛋白的组织受到损害。电化学方法，特别是电化学阻抗谱（EIS）用于评估物理化学特性，阻抗曲线显示出对材料表面粗糙度的依赖性。使用等效电路对EIS结果进行进一步分析，该等效电路对通过接口的电流进行建模。获得了来自具有表面性质的等效电路的参数之间的相关性的第一指征，其有望与细胞的生物学反应相关。

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