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Multiple parameter cytotoxicity index on dental alloys and pure metals

机译：对牙科合金和纯金属的多参数细胞毒性指数

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摘要

Palladium (Pd) is a metal frequently used for dental alloys. In order to elucidate controversial options about Pd concerning its biological performances, our study consists in the evaluation of commercial and experimental PFM and C&B precious and semi-precious dental alloys. This investigation was also designated to the establishment of a cytotoxicity index (CI) such as it was described for hemocompatibility testing. The following materials were tested: 36 commercial alloys (Au-, Pd- and Ag-base), 14 experimental alloys (Pd-base established by an experience plan) and pure metals (Ag, Au, Cu, Ni, Cr, In, Sn, Pt, Ti, Zn). The cells culture experiments were carried out with epithelial L132 cells and NIH 3T3 fibroblasts. In vitro cell viability tests show that Pt, Sn, In, Ti, Au and Pd have no cytotoxic effect; Cr, Cu and Ag are toxic, Ni, Zn, and Co are highly toxic. An identical ranking was found with the inflammatory and proliferation tests. Toxic and highly toxic metals induced slight or strong prosthetic dental restoration morphological alterations after 3-days cultures and mostly cell death after 6-days cultures. These effects are dependent on the leakage of the element into the culture medium as revealed by ICP. The addition of Au gives benefit to Pd-Ag alloys, but does not produce any major effect on Pd-Cu alloys. This qualitative ranking can quantitatively be confirmed by cytocompatibility testing after application of a CI.
机译：钯（Pd）是经常用于牙科合金的金属。为了阐明有关Pd生物学性能的争议性选择，我们的研究包括对商用和实验性PFM和C＆B贵金属和半贵金属合金的评估。该研究还被指定用于建立细胞毒性指数（CI），例如用于血液相容性测试的描述。测试了以下材料：36种市售合金（Au，Pd和Ag基），14种实验合金（根据经验计划建立Pd基）和纯金属（Ag，Au，Cu，Ni，Cr，In， Sn，Pt，Ti，Zn）。用上皮L132细胞和NIH 3T3成纤维细胞进行细胞培养实验。体外细胞生存力测试表明，Pt，Sn，In，Ti，Au和Pd没有细胞毒性作用。 Cr，Cu和Ag有毒，Ni，Zn和Co有剧毒。在炎症和增殖测试中发现了相同的排名。有毒和剧毒的金属在培养3天后诱导轻微或强烈的义齿修复牙齿形态变化，在培养6天后主要导致细胞死亡。这些影响取决于ICP所揭示的元素向培养基中的泄漏。 Au的添加使Pd-Ag合金受益，但对Pd-Cu合金没有任何重大影响。在应用CI后，可通过细胞相容性测试定量确定该定性排名。

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