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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >UV Raman Determination of the Environment and Solvent Exposure of Tyr and Trp Residues
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UV Raman Determination of the Environment and Solvent Exposure of Tyr and Trp Residues

机译：紫外拉曼光谱法测定Tyr和Trp残留物的环境和溶剂暴露量

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摘要

We characterized the dependence of the 229 nm Raman cross sections of tyr and trp on solvent exposure in native horse and tuna cytochrome c and in horse and sperm whale myoglobin. Tyr and trp residues with larger solvent accessible surface areas have smaller 229 nm excited tyr and trp Raman cross sections. We observe that in water-propanol solvent mixtures the 229 nm excited tyr and trp Raman cross sections increase with decreasing water composition due to red shifts of the ～220 nm tyr and trp absorption bands. The tyr and trp protein Raman cross section changes result from absorption red shifts for environments with increasing hydrogen bond donor basicity, decreasing hydrogen bond acceptor acidity, and increasing polarity. We empirically correlate changes in Raman cross sections with the tyr and trp residue solvent accessibility. The Raman cross sections of tyr and trp residues can now be quantitatively utilized to probe local protein environments.
机译：我们表征了tyr和trp的229 nm拉曼横截面对天然马和金枪鱼细胞色素c以及马和抹香鲸肌红蛋白中溶剂暴露的依赖性。具有较大溶剂可及表面积的Tyr和trp残留物具有更小的229 nm激发的tyr和trp拉曼横截面。我们观察到在水-丙醇溶剂混合物中，由于〜220 nm轮胎和trp吸收带的红移，229 nm激发的轮胎和trp拉曼截面随水组成的减少而增加。 tyr和trp蛋白拉曼横截面的变化是由于在氢键供体碱度增加，氢键受体酸度降低和极性增加的环境中吸收红移引起的。我们根据经验将拉曼横截面的变化与tyr和trp残留溶剂的可及性联系起来。 tyr和trp残基的拉曼横截面现在可以定量地用于探测局部蛋白质环境。

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