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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Combined Small-Angle X-ray and Neutron Scattering Experiments for Thickness Characterization of Ganglioside Bilayers
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Combined Small-Angle X-ray and Neutron Scattering Experiments for Thickness Characterization of Ganglioside Bilayers

机译：小角度X射线和中子散射相结合的神经节苷脂双层厚度表征实验

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摘要

Combined small-angle neutron and X-ray Scattering experiments on dilute solutions of the ganglioside GM3 in water are presented. The contrast profiles along the lipid and sugar portions of the ganglioside molecule for the two types of radiations are different, a feature which, added to the extension of the scattering q range to q ～ 0.4 A~(-1), allows an accurate determination of the cross section of the bilayer in dilute solution. The electronic density profile of the bilayer cross section can be interpreted only by assuming thickness fluctuations, which have already been theoretically predicted for the GM3 bilayers, due to the peculiar packing properties of the monomer. Increasing temperature from room temperature to 50 ℃ has the effect of flattening the GM3 bilayer, as the thickness and the water content of the outer hydrophilic layer decrease, while the thickness of the inner hydrophobic region is almost unchanged.
机译：结合小角度中子和X射线散射实验对神经节苷脂GM3在水中的稀释溶液进行了研究。两种辐射沿神经节苷脂分子的脂质和糖部分的对比度分布是不同的，这一特征加到散射q范围扩展到q〜0.4 A〜（-1）上，可以精确测定稀释溶液中双层横截面的横截面图。双层横截面的电子密度分布只能通过假设厚度波动来解释，这是由于单体的特殊堆积特性而在理论上已经对GM3双层进行了预测。将温度从室温升高到50℃具有使GM3双层变平的效果，因为外部亲水层的厚度和含水量减小，而内部疏水区的厚度几乎不变。

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