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首页> 外文期刊>The journal of physical chemistry, C. Nanomaterials and interfaces >Structural and Functional Characterization of Enzyme-Quantum Dot Conjugates: Covalent Attachment of CdS Nanocrystal to -Chymotrypsin
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Structural and Functional Characterization of Enzyme-Quantum Dot Conjugates: Covalent Attachment of CdS Nanocrystal to -Chymotrypsin

机译：酶-量子点缀合物的结构和功能表征：CdS纳米晶体与胰凝乳蛋白酶的共价结合。

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摘要

In this contribution, we report the synthesis of CdS nanoparticles directly conjugated to an enzyme, -chymotrypsin (CHT) by chemical reduction in aqueous solution. Matrix-assisted laser desorption ionization (MALDI) mass spectrometry confirms the strong conjugation of CdS nanoparticles to CHT through covalent bonding in the CdS-CHT conjugates. High-resolution transmission electron microscopy (HRTEM) reveals that the CdS nanoparticles are well dispersed with an average diameter of 3 nm, and energy dispersive X-ray elemental analysis (EDAX) verifies the composition of the CdS-protein conjugates. The structural integrity of CHT before and after the conjugation of CdS nanoparticles is confirmed through circular dichroism (CD) studies. The functional integrity of CHT conjugated to CdS nanoparticles is investigated by monitoring the enzymatic activities of CHT and CHT-conjugated CdS nanoparticles by using absorption spectroscopy. The attachment of CdS nanoparticles to an enzyme with its structural and functional integrity may have impact in the future research on nanobio interface.
机译：在这项贡献中，我们报告了通过水溶液中的化学还原直接与酶-胰凝乳蛋白酶（CHT）偶联的CdS纳米颗粒的合成。基质辅助激光解吸电离（MALDI）质谱证实了CdS-CHT共轭物中CdS纳米粒子通过共价键与CHT的强共轭。高分辨率透射电子显微镜（HRTEM）显示CdS纳米颗粒分散良好，平均直径为3 nm，并且能量色散X射线元素分析（EDAX）验证了CdS-蛋白质偶联物的组成。通过圆二色性（CD）研究证实了CdS纳米粒子结合前后CHT的结构完整性。通过使用吸收光谱法监测CHT和CHT共轭CdS纳米粒子的酶活性，研究了共轭CdS纳米粒子与CHT的功能完整性。 CdS纳米粒子与酶的结合及其结构和功能的完整性可能会对纳米生物界面的未来研究产生影响。

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