Targeted cDNA differential display (TcDD)

Nguyen GH; Nguyen NC; Storm N; Gange C; Gavras H; Smith CL

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Targeted cDNA differential display (TcDD)

机译：靶向cDNA差异显示（TcDD）

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摘要

Targeted cDNA differential display (TcDD) was developed to study expression of a different selected gene families especially those at low copy numbers per cell. This method is an adaptation of our previously described targeted genomic differential display method (TGDD). In TcDD, the expression of genes containing target sequences such as CAG repeating sequences or genes encoding for zinc-finger binding proteins were followed in an experimental rat model with salt-induced hypertension. DNA sequencing experiments demonstrated that the effectiveness of targeting was greater than 99%. (C) 2006 Elsevier B.V. All rights reserved.

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《Biomolecular engineering》 |2006年第1期|共14页
作者
Nguyen GH; Nguyen NC; Storm N; Gange C; Gavras H; Smith CL;
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正文语种 eng
中图分类遗传学;
关键词
differential display; cDNA; gene expression; gene families; repeats; MESSENGER-RNA; GENE-EXPRESSION; MOUSE TISSUES; PCR; IDENTIFICATION; SEQUENCE; REPEATS; BRAIN; CELLS; DNA;

机译：差异显示;cDNA;基因表达;基因家族;重复;MESSENGER-RNA;基因表达;小鼠组织;PCR;鉴定;序列;重复;脑;细胞;DNA;

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专利

1. Targeted cDNA differential display (TcDD) [J] . Nguyen GH, Nguyen NC, Storm N, Biomolecular engineering . 2006,第1期

机译：靶向cDNA差异显示（TcDD）
2. Combined screening of thymocytes using apoptosis-specific cDNA array and promoter analysis yields novel gene targets mediating TCDD-induced toxicity. [J] . Fisher MT, Nagarkatti M, Nagarkatti PS Toxicological sciences: An official journal of the Society of Toxicology . 2004,第1期

机译：使用凋亡特异性cDNA阵列和启动子分析相结合的胸腺细胞筛选产生介导TCDD诱导毒性的新型基因靶标。
3. PG25, a pineal-specific cDNA, cloned by differential display PCR (DDPCR) and rapid amplification of cDNA ends (RACE). [J] . Wang X, Brownstein MJ, Young WS Journal of Neuroscience Methods . 1997,第2期

机译：PG25是一种松果体特异性cDNA，可通过差异显示PCR（DDPCR）和cDNA末端的快速扩增（RACE）进行克隆。
4. Identification of candidate cDNAs correlated to malting quality of barley by means of cDNA-AFLP analysis and differential genotype pooling [C] . Markus Herz, Sabine Mikolajewski, Günther Schweizer, EBC congress;Dublin 2003 . 2003

机译：通过cDNA-AFLP分析和差异基因型合并鉴定与大麦发芽品质相关的候选cDNA
5. Characterization ofp53 target genes identified by differential display: TIS11D and CYFIP2. [D] . Jackson, Roger Stanley, II. 2007

机译：通过差异显示鉴定的p53靶基因的表征：TIS11D和CYFIP2。
6. Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of northern blot for affinity capturing of cDNAs. [O] . F Li, E S Barnathan, K Karikó 1994

机译：快速筛选和克隆在差异mRNA显示中产生的cDNA的快速方法：应用Northern印迹法亲和捕获cDNA。
7. Combined Screening of Thymocytes Using Apoptosis-Specific cDNA Array and Promoter Analysis Yields Novel Gene Targets Mediating TCDD-Induced Toxicity [O] . M. T. Fisher 2004

机译：使用凋亡特异性cDNA阵列和启动子分析的组合筛选胸腺细胞产量介导TCDD诱导毒性的新型基因靶标
8. Phage Display Breast Carcinoma cDNA Libraries: Isolation of Clones Which Specifically Bind to Membrane Glycoproteins, Mucins, and Endothelial Cell Surface [R] . Yamamoto, F. 2001

机译：噬菌体展示乳腺癌cDNa文库：分离与膜糖蛋白，粘蛋白和内皮细胞表面特异性结合的克隆

Targeted cDNA differential display (TcDD)

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