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首页> 外文期刊>The Journal of Nutritional Biochemistry >Maternal postnatal high-fat diet, rather than gestational diet, affects morphology and mTOR pathway in skeletal muscle of weaning rat.
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Maternal postnatal high-fat diet, rather than gestational diet, affects morphology and mTOR pathway in skeletal muscle of weaning rat.

机译：产后高脂饮食而不是妊娠饮食会影响断奶大鼠骨骼肌的形态和mTOR途径。

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The positive regulation of insulin pathway in skeletal muscle results in increased activity of the mammalian target of rapamycin (mTOR), a positive effector of mRNA translation rate and protein synthesis. Studies that assess the activity of this protein in response to chronic high-fat diet (HFD) are scarce and controversial, and to date, there are no studies evaluating the mTOR pathway in infants exposed to gestational and postgestational HFD. This study investigated the effect of maternal HFD on skeletal muscle morphology and on phosphorylation of proteins that comprise the intracellular mTOR signaling pathway in soleus muscle of offspring at weaning. For this purpose, 10 days prior to conception, 39 female Wistar rats were randomly assigned to either control diet (CTL) or HFD. Later, rats were distributed into four groups according to gestational and postpregnancy diet: CTL/CTL (n=10), CTL/HF (n=11), HF/HF (n=10) and HF/CTL (n=8). After 21 days of lactation, pups were killed, and blood samples and soleus and gastrocnemius skeletal muscle were collected for analysis. We observed an influence of maternal postgestational diet, rather than gestational diet, in promoting an obese phenotype, characterized by body fat accumulation, insulin resistance and high serum leptin, glucose, triglycerides and cholesterol levels (P<.05). We have also detected alterations on skeletal muscle morphology - with reduced myofiber density - and impairment on S6 kinase 1 and 4E binding protein-1 phosphorylation (P<.05). These results emphasize the importance of maternal diet during lactation on muscle morphology and on physiological adaptations of infant rats
机译：骨骼肌中胰岛素途径的正向调节导致哺乳动物雷帕霉素靶标（mTOR）的活性增加，而雷帕霉素（mTOR）是mRNA翻译率和蛋白质合成的积极效应物。评估这种蛋白质对慢性高脂饮食（HFD）的反应的研究稀少且有争议，迄今为止，尚无评估暴露于妊娠和妊娠HFD的婴儿中mTOR途径的研究。这项研究调查了母体HFD对断奶后代比目鱼肌中骨骼肌形态和蛋白质表达的影响，这些蛋白质包括细胞内mTOR信号通路。为此，在受孕前10天，将39只Wistar雌性大鼠随机分为对照饮食（CTL）或HFD。之后，根据妊娠和孕后饮食将大鼠分为四组：CTL / CTL（n = 10），CTL / HF（n = 11），HF / HF（n = 10）和HF / CTL（n = 8） 。哺乳21天后，将幼崽处死，收集血样，比目鱼肌和腓肠肌骨骼肌进行分析。我们观察到孕妇的孕期饮食而不是孕期饮食对促进肥胖表型的影响，肥胖表型的特征在于体脂肪积累，胰岛素抵抗和高血清瘦素，葡萄糖，甘油三酸酯和胆固醇水平（P <0.05）。我们还检测到骨骼肌形态发生改变-肌纤维密度降低-以及S6激酶1和4E结合蛋白1磷酸化受损（P <.05）。这些结果强调了哺乳期间母体饮食对婴儿的肌肉形态和生理适应性的重要性。

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