Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta.

Maldonado LM; Hernandez VE; Rivero EM; Barba-de-la-Rosa AP; Flores JL; Acevedo LG; De-Leon-Rodriguez A

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Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta.

机译：使用响应面方法优化大肠杆菌中合成基因表达的培养条件：人干扰素β的情况。

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A human interferon beta (hINF-beta) synthetic gene was optimized and expressed in Escherichia coli BL21-SI using a vector with the T7 promoter. To determine the best culture conditions such as culture medium, temperature, cell density and inducer concentration, we used the response surface methodology and a Box-Behnken design to get the highest hINF-beta production. The maximum hINF-beta production of 61 mg l(-1) was attained using minimum medium and the following predicted optimal conditions: temperature of 32.5 degrees C, cell density of 0.64, and inducer concentration of 0.30 M NaCl. This is the first report showing the successful performance of the BL21-SI system in a minimum medium. The response surface methodology is effective for the optimization of recombinant protein production using synthetic genes.

机译：使用具有T7启动子的载体，优化了人干扰素beta（hINF-beta）合成基因并在大肠杆菌BL21-SI中表达。为了确定最佳的培养条件，例如培养基，温度，细胞密度和诱导剂浓度，我们使用了响应面方法和Box-Behnken设计来获得最高的hINF-beta产量。使用最小培养基和以下预测的最佳条件，可获得最大的hINF-beta产量61 mg l（-1）：温度为32.5摄氏度，细胞密度为0.64，诱导剂浓度为0.30 M NaCl。这是第一份报告，显示了BL21-SI系统在最小介质中的成功性能。响应面方法对于使用合成基因优化重组蛋白生产非常有效。

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《Biomolecular engineering》 |2007年第2期|共6页
作者
Maldonado LM; Hernandez VE; Rivero EM; Barba-de-la-Rosa AP; Flores JL; Acevedo LG; De-Leon-Rodriguez A;
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正文语种 eng
中图分类遗传学;医用一般科学;
关键词
methodology lt; 2gt; Cultural; Production; Human; Interferon-beta; 干扰素β;

机译：methodology 2;Cultural;Production;Human;Interferon-beta;干扰素β;

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1. Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta. [J] . Maldonado LM, Hernandez VE, Rivero EM, Biomolecular engineering . 2007,第2期

机译：使用响应面方法优化大肠杆菌中合成基因表达的培养条件：人干扰素β的情况。
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6. Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology [O] . Sri Agung Fitri Kusuma, Ida Parwati, Tina Rostinawati, 2019

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7. Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology [O] . Sri Agung Fitri Kusuma, Ida Parwati, Tina Rostinawati, 2019

机译：使用表面响应方法，对大肠杆菌BL21（DE3）中MPT64合成基因表达的培养条件优化

Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta.

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