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首页> 外文期刊>The Journal of Nutritional Biochemistry >Niacin deficiency causes oxidative stress in rat bone marrow cells but not through decreased NADPH or glutathione status.
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Niacin deficiency causes oxidative stress in rat bone marrow cells but not through decreased NADPH or glutathione status.

机译：烟酸缺乏会在大鼠骨髓细胞中引起氧化应激，但不会通过降低NADPH或谷胱甘肽状态而引起。

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摘要

Niacin (vitamin B(3)), in the form of NADPH, is required for the regeneration of glutathione (GSH), which is the substrate of GSH peroxidase. In this study, we examined the effect of dietary niacin deficiency on protein and DNA oxidation in bone marrow cells of Long-Evans rats. Western blotting was used to measure 2,4-dinitrophenylhydrazine-reactive protein carbonyl products, and the Biotrin OxyDNA method was used to measure 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). The levels of both protein carbonyls and 8-oxodG were increased by 50% in niacin-deficient bone marrow cells. To examine whether this oxidant damage involves altered metabolism of pyridine nucleotides and glutathione, both oxidized and reduced forms of pyridine nucleotides (NAD(+), NADH, NADP(+), NADPH) and glutathione (GSSG and GSH) were quantified in total and nucleated bone marrow cells. NAD and NADP(+) levels were decreased 80% and 22%, respectively, by niacin deficiency. NADPH and GSH were not depleted by niacin deficiency, showing that oxidant injury was not due directly to impairment of this pathway. Oxidative stress, of uncertain etiology, may play a role in the observed genomic instability and sensitivity to leukemogenesis in bone marrow cells during niacin deficiency.
机译：烟酸（NADPH形式的维生素B（3））是谷胱甘肽（GSH）（GSH过氧化物酶的底物）的再生所必需的。在这项研究中，我们检查了饮食中烟酸缺乏对Long-Evans大鼠骨髓细胞蛋白质和DNA氧化的影响。 Western印迹法用于测定2,4-二硝基苯肼反应性蛋白羰基产物，Biotrin OxyDNA方法用于测定8-oxo-7,8-dihydro-2'-deoxyguanosine（8-oxodG）。在烟酸缺乏的骨髓细胞中，蛋白质羰基和8-oxodG的水平均增加了50％。为了检查这种氧化剂损伤是否涉及吡啶核苷酸和谷胱甘肽的代谢改变，对吡啶核苷酸（NAD（+），NADH，NADP（+），NADPH）和谷胱甘肽（GSSG和GSH）的氧化形式和还原形式进行了定量分析，有核骨髓细胞。由于烟酸缺乏，NAD和NADP（+）水平分别降低了80％和22％。 NADPH和GSH并没有因烟酸缺乏而耗尽，这表明氧化损伤不是直接由于该途径的损伤而引起的。病因不明的氧化应激可能在烟酸缺乏期间观察到的基因组不稳定性和骨髓细胞对白血病发生的敏感性中起作用。

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