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首页> 外文期刊>The Journal of Nutritional Biochemistry >Reversal of chemical-induced liver fibrosis in Wistar rats by puerarin
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Reversal of chemical-induced liver fibrosis in Wistar rats by puerarin

机译：葛根素逆转Wistar大鼠化学性肝纤维化

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摘要

Puerarin is a major isoflavonoid compound isolated from Pueraria lobata, an edible vine used widely for various medicinal purposes. It has been used for centuries in China to counteract alcohol intoxication. However, the effects of puerarin on chemical-induced liver fibrosis have not been reported. In the present study, we investigated the effects of puerarin on liver fibrosis in Wistar rats induced by alcohol plus carbon tetrachloride administration. Liver fibrosis was produced in rats by treatment with a mixture (50% alcohol, 8 g/kg per day; corn oil, 2 g/kg per day; pyrazole, 24 mg/kg per day; ig) once a day and by intraperitoneal injection of 0.25 ml/kg of a 25% solution of carbon tetrachloride in olive oil twice a week for 8 weeks. After 8 weeks, treatment with puerarin (0.4 and 0.8 g/kg ig, daily for 4 weeks) was conducted to examine its therapeutic effects. At the same time, the model group and treatment group continued to receive the chemical mixture, while the control group received saline instead of the chemical mixture. Upon pathological examination, the puerarin-treated rats significantly reversed the symptoms of liver fibrosis and other hepatic lesions. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as indexes of hepatic cell disruption, were reduced with puerarin treatment, whereas no significant effect was discovered in the levels of alkaline phosphatase (ALP) and gamma-glutamyltransferase (GGT) activities. A significant increase in apoptosis of activated hepatic stellate cell (HSC) was found by flow cytometric analysis of the hepatic tissues. And the expression of bcl-2 mRNA was down-regulated after puerarin administration. Consequently, all these results showed that puerarin could effectively reverse chemical-induced liver fibrosis in experimental rats, via the recovery of hepatic injury as well as the induction of apoptosis in activated HSC.
机译：葛根素是从葛根（Pueraria lobata）中分离出来的一种主要的异黄酮化合物，葛根是一种可食用的葡萄藤，广泛用于各种医学用途。在中国，它已经被使用了几个世纪来抵抗酒精中毒。然而，葛根素对化学性肝纤维化的作用尚未见报道。在本研究中，我们调查了葛根素对酒精加四氯化碳诱导的Wistar大鼠肝纤维化的影响。每天一次用混合物（50％酒精，每天8 g / kg;玉米油，每天2 g / kg;吡唑，每天24 mg / kg; ig）处理，在大鼠中产生肝纤维化。每周两次注射0.25 ml / kg的25％四氯化碳在橄榄油中的溶液，持续8周。 8周后，进行葛根素治疗（0.4和0.8 g / kg ig，每天4周），以检查其治疗效果。同时，模型组和治疗组继续接受化学混合物，而对照组则接受生理盐水代替化学混合物。经病理检查，葛根素治疗的大鼠显着逆转了肝纤维化和其他肝损害的症状。葛根素治疗可降低血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）作为肝细胞破坏的指标，而碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）活性水平未见明显影响。通过肝组织的流式细胞术分析发现活化的肝星状细胞（HSC）的凋亡显着增加。葛根素给药后bcl-2 mRNA的表达下调。因此，所有这些结果表明，葛根素可以通过恢复肝损伤以及诱导活化的HSC凋亡来有效逆转实验性化学诱导的大鼠肝纤维化。

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