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首页> 外文期刊>The Journal of Nutritional Biochemistry >Soy isoflavones increase quinone reductase in hepa-1c1c7 cells via estrogen receptor beta and nuclear factor erythroid 2-related factor 2 binding to the antioxidant response element.
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Soy isoflavones increase quinone reductase in hepa-1c1c7 cells via estrogen receptor beta and nuclear factor erythroid 2-related factor 2 binding to the antioxidant response element.

机译：大豆异黄酮通过雌激素受体β和核因子红系2相关因子2与抗氧化反应元件的结合来增加hepa-1c1c7细胞中的醌还原酶。

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Soy protein and isoflavones (genistein and daidzein) have been demonstrated to increase quinone reductase (QR) activity, protein, and mRNA in animal and cell culture models. However, their mechanism of action has not been completely characterized. Additionally, it has not been determined if equol, a daidzein metabolite, can modulate QR activity and expression. Estrogen receptor beta (ER beta) is thought to be involved in stimulating QR gene transcription by anti-estrogens and phytoestrogens, along with nuclear factor erythroid 2-related factor 2 (Nrf2). This study tested the hypothesis that genistein, daidzein and equol increase quinone reductase activity, protein and mRNA via ER beta and Nrf2 binding to the QR antioxidant response element (ARE). QR expression and activity were determined using TaqMan polymerase chain reaction, protein immunoblots and activity assays. Molecular events were investigated using luciferase reporter gene assays and chromatin immunoprecipitation (ChIP). Hepa-1c1c7 cells were treated with control (0.1% (v:v) dimethyl sulfoxide (DMSO)); 1 mumol/L beta-naphthoflavone (positive control); 5 mumol/L resveratrol (ChIP positive control for ER beta binding) and 1, 5 and 25 mumol/L genistein, daidzein or equol. Treatment durations were 1 h (ChIP), 24 h (mRNA and luciferase assays) and 24 and 48 h (protein and activity). Genistein, daidzein and equol increased QR activity, protein and mRNA, with daidzein and equol having more of an impact at physiologic concentrations (1 and 5 mumol/L) compared to genistein. Furthermore, the study results demonstrate that genistein, daidzein and equol interact with the QR ARE and that daidzein and equol act via both ER beta and Nrf2 binding strongly to the QR ARE
机译：在动物和细胞培养模型中，大豆蛋白和异黄酮（染料木黄酮和黄豆苷元）已被证明可以增加醌还原酶（QR）活性，蛋白和mRNA。但是，它们的作用机理尚未完全表征。另外，尚未确定雌黄酮的代谢产物雌马酚是否可调节QR活性和表达。雌激素受体β（ER beta）被认为参与抗雌激素和植物雌激素以及核因子红系2相关因子2（Nrf2）刺激QR基因的转录。这项研究验证了染料木黄酮，黄豆苷元和雌马酚通过ER beta和Nrf2与QR抗氧化反应元件（ARE）结合而提高醌还原酶活性，蛋白质和mRNA的假设。使用TaqMan聚合酶链反应，蛋白质免疫印迹和活性测定法确定QR表达和活性。使用萤光素酶报告基因测定和染色质免疫沉淀（ChIP）研究分子事件。用对照（0.1％（v：v）二甲基亚砜（DMSO））处理Hepa-1c1c7细胞; 1μmol/ Lβ-萘黄酮（阳性对照）; 5 mumol / L白藜芦醇（ERβ结合的ChIP阳性对照）和1、5和25 mumol / L染料木黄酮，黄豆苷元或雌马酚。治疗时间为1小时（ChIP），24小时（mRNA和荧光素酶测定）以及24和48小时（蛋白质和活性）。与染料木黄酮相比，染料木黄酮，大豆苷元和雌马酚可增加QR活性，蛋白质和mRNA，其中大豆苷元和牛尿酚对生理浓度（1和5μmol/ L）的影响更大。此外，研究结果表明金雀异黄素，黄豆苷元和雌马酚与QR ARE相互作用，并且黄豆苷元和雌马酚通过ER beta和Nrf2均强烈结合QR ARE

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