• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Nutritional Biochemistry >Resveratrol down-regulates interferon- gamma-inducible inflammatory genes in macrophages: molecular mechanism via decreased STAT-1 activation.
【24h】

Resveratrol down-regulates interferon- gamma-inducible inflammatory genes in macrophages: molecular mechanism via decreased STAT-1 activation.

机译：白藜芦醇下调巨噬细胞中干扰素-γ诱导的炎症基因：STAT-1激活降低的分子机制。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Resveratrol (trans-3,4',5-trihydroxystilbene) is one of nonflavonoid polyphenolic phytoalexins found in various plant species, a number of which are components of human diet including grapes and red wines. Resveratrol has exerted several beneficial effects with anti-inflammation, cardioprotection and cancer chemoprevention. However, its mechanisms of action are not completely understood. In this study, we investigated effects of resveratrol on inflammatory gene expression in interferon (IFN)- gamma alone-stimulated macrophages and proposed a molecular basis underlying the action. Resveratrol inhibited IFN- gamma-induced production of nitric oxide (NO), IFN- gamma-inducible protein-10 (IP-10), or the monokine induced by IFN- gamma (MIG) in RAW 264.7 macrophages and also that of NO in primary macrophages derived from bone marrows of C3H/HeJ (toll-like receptor-4--/--) mice. Moreover, resveratrol diminished IFN- gamma-induced protein levels of inducible NO synthase (iNOS), attenuated mRNA levels of iNOS, IP-10 or MIG as well as inhibited IFN- gamma-induced promoter activity of iNOS gene, indicating that the phytoalexin could down-regulate inflammatory genes at the transcription level. To understand a mechanism of the action, we tested resveratrol could affect the signal transducers and activation of transcription-1 (STAT-1), a pivotal transcription factor in IFN- gamma-induced expression of inflammatory genes. Resveratrol inhibited IFN- gamma-induced transcriptional activity of STAT-1 in macrophages and also IFN- gamma-induced Tyr701 or Ser727 phosphorylation of STAT-1. We then focused on protein kinases upstream STAT-1 phosphorylation. Resveratrol inhibited IFN- gamma-induced activation of Janus kinase-2 (JAK-2) and also the extracellular signal-regulated kinase, in which JAK-2 was more sensitive. Taken together, this study proposes a new mechanism of resveratrol, blocking JAK/STAT-1 pathway that controls inflammatory responses in IFN- gamma-activated macrophages. All rights reserved, Elsevier.
机译：白藜芦醇（反式-3,4'，5-三羟基sti）是一种在各种植物中发现的非黄酮类多酚类植物抗毒素，其中许多是人类饮食中的成分，包括葡萄和红酒。白藜芦醇在消炎，心脏保护和癌症化学预防方面发挥了多种有益作用。但是，其作用机理尚未完全了解。在这项研究中，我们调查了白藜芦醇对干扰素（IFN）-γ单独刺激的巨噬细胞中炎性基因表达的影响，并提出了该作用的分子基础。白藜芦醇抑制RAW 264.7巨噬细胞中IFN-γ诱导的一氧化氮（NO），IFN-γ诱导性蛋白10（IP-10）或IFN-γ（MIG）诱导的单核因子的生成，以及原代巨噬细胞来自C3H / HeJ（toll-like receptor-4 -/-）小鼠的骨髓。此外，白藜芦醇降低了IFN-γ诱导的一氧化氮合酶（iNOS）的蛋白水平，减弱了iNOS，IP-10或MIG的mRNA水平，并抑制了IFN-γ诱导的iNOS基因的启动子活性，表明该植物抗毒素可以在转录水平下调炎症基因。为了了解其作用机理，我们测试了白藜芦醇可能会影响信号转导和转录1（STAT-1）的激活，转录1（STAT-1）是IFN-γ诱导的炎症基因表达的关键转录因子。白藜芦醇抑制IFN-γ诱导的巨噬细胞STAT-1的转录活性，也抑制IFN-γ诱导的Tyr 701 或Ser 727 的STAT-1磷酸化。然后，我们专注于上游STAT-1磷酸化的蛋白激酶。白藜芦醇抑制了IFN-γ诱导的Janus激酶2（JAK-2）的活化，也抑制了细胞外信号调节激酶，其中JAK-2更为敏感。综上所述，这项研究提出了白藜芦醇的新机制，该机制阻断了控制IFN-γ活化巨噬细胞中炎症反应的JAK / STAT-1途径。保留所有权利，Elsevier。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号