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首页> 外文期刊>The Journal of Nutritional Biochemistry >Anti-obesity effect of carboxymethyl chitin by AMPK and aquaporin-7 pathways in 3T3-L1 adipocytes
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Anti-obesity effect of carboxymethyl chitin by AMPK and aquaporin-7 pathways in 3T3-L1 adipocytes

机译：羧甲基几丁质通过AMPK和aquaporin-7途径在3T3-L1脂肪细胞中的抗肥胖作用

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摘要

The aim of this study was to investigate the anti-obesity effect of carboxymethyl-chitin (CM-chitin). a water-soluble derivative of chitin, by measuring lipid accumulation and adipogenesis related factors in 3T3-L1 adipocytes. CM-chitin was synthesized by means of carboxymethylation reaction. Its inhibitory effect on lipid accumulation was investigated by measuring triglyceride content and glycerol release level. The gene and protein levels associated with adipogenesis were determined using reverse transcriptase-polymerase chain reaction and Western blot analysis. Treatment with CM-chitin reduced triglyceride content and enhanced glycerol secretion in a dose-dependent manner. CM-chitin induced the down-regulation of adipogenesis related transcriptional factors and adipocyte specific gene promoters. Moreover, the specific mechanism by CM-chitin was confirmed by transcriptional activations of the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and aquaporin-7. These results suggest that CM-chitin exerts anti-adipogenic effect on lipid accumulation through modulations of AMPK and aquaporin-7 signal pathways
机译：这项研究的目的是研究羧甲基壳多糖（CM-几丁质）的抗肥胖作用。通过测量3T3-L1脂肪细胞中的脂质蓄积和脂肪形成相关因子来获得几丁质的水溶性衍生物。 CM-几丁质通过羧甲基化反应合成。通过测量甘油三酸酯含量和甘油释放水平来研究其对脂质积累的抑制作用。使用逆转录酶-聚合酶链反应和蛋白质印迹分析确定与脂肪形成相关的基因和蛋白质水平。 CM-甲壳质的治疗以剂量依赖的方式降低了甘油三酯的含量并增加了甘油的分泌。 CM-几丁质诱导脂肪形成相关转录因子和脂肪细胞特异性基因启动子的下调。此外，CM-几丁质的特异性机制通过磷酸化的磷酸腺苷单磷酸激活的蛋白激酶（AMPK）和水通道蛋白7的转录激活得到证实。这些结果表明，CM-甲壳质通过调节AMPK和aquaporin-7信号通路对脂质积累发挥抗脂肪形成作用。

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