• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Nutritional Biochemistry >Polyunsaturated fatty acids incorporation into cardiolipin in H9c2 cardiac myoblast
【24h】

Polyunsaturated fatty acids incorporation into cardiolipin in H9c2 cardiac myoblast

机译：H9c2心肌成肌细胞中多不饱和脂肪酸并入心磷脂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), known as omega-3 polyunsaturated fatty acid (PUFA), are common nutrients in daily food intake and have been shown to prevent cardiovascular disease and improve cardiac functions. Cardiolipin is a mitochondrial phospholipid necessary for maintaining physiological function of mitochondria. Several studies have indicated that the cardiolipin acyl chain compositions affect the function of cardiolipin and mitochondria. Here, we investigated the structural changes of cardiolipin after DHA and EPA supplementation and compared them to arachidonic acid (AA) treatment. H9c2 cardiac myoblast was used as a cell model, and cardiolipin species was monitored and identified via LC-MS and MS/MS. Our results showed distinct mass envelopes of cardiolipin with the same carbon number but different double bonds in mass spectrum. There were 116 cardiolipin species with 36 distinct mass in 6 mass envelopes identified by MS/MS. Three days of PUFA treatment resulted in decreases of low-molecular-weight cardiolipin and increases of high-molecular-weight cardiolipin, suggesting the incorporation of exogenous DHA, EPA and AA into mitochondrial cardiolipin. PUFA incorporation was further verified by MS/MS analysis. More importantly, we found that DHA supplementation elevated the percent content of less unsaturated cardiolipin species and highly unsaturated cardiolipin species, containing omega-3 fatty acyl chains, indicating a omega-3 fatty acid incorporation mechanism with peroxidation protection. Our results indicate that PUFA supplementation differentially perturbed the fatty acyl chain compositions in the mitochondrial cardiolipin in the H9c2 cardiac myoblast, suggesting that mitochondrial membrane and the function of mitochondria are susceptible to exogenous lipid species. (C) 2015 Elsevier Inc. All rights reserved.
机译：二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）被称为omega-3多不饱和脂肪酸（PUFA），是日常食物摄入中的常见营养素，并已被证明可以预防心血管疾病和改善心脏功能。心磷脂是维持线粒体生理功能所必需的线粒体磷脂。多项研究表明，心磷脂酰基链组成会影响心磷脂和线粒体的功能。在这里，我们调查了DHA和EPA补充后心磷脂的结构变化，并将其与花生四烯酸（AA）处理进行了比较。 H9c2心脏成肌细胞用作细胞模型，并通过LC-MS和MS / MS监测和鉴定心磷脂种类。我们的研究结果显示，具有相同碳数但在质谱中具有不同双键的心磷脂具有不同的质量包膜。通过MS / MS鉴定，在6个质量包膜中有116种心磷脂物种具有36种不同的质量。 PUFA治疗三天导致低分子量心磷脂减少，而高分子量心磷脂增加，表明线粒体心磷脂中掺入了外源DHA，EPA和AA。通过MS / MS分析进一步验证了PUFA的掺入。更重要的是，我们发现DHA的添加提高了含有omega-3脂肪酰基链的较少不饱和心磷脂和高度不饱和心磷脂的百分含量，表明具有过氧化保护作用的omega-3脂肪酸掺入机制。我们的结果表明，PUFA补充剂会不同程度地干扰H9c2心肌成肌细胞线粒体心磷脂中的脂肪酰基链组成，这表明线粒体膜和线粒体的功能易受外源脂质种类的影响。 （C）2015 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号