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首页> 外文期刊>The journal of obstetrics and gynaecology research >Molecular investigation of menstrual tissue for the presence of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis collected by women with a history of infertility
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Molecular investigation of menstrual tissue for the presence of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis collected by women with a history of infertility

机译：女性不育病史收集的月经组织中沙眼衣原体，解脲脲原体和人支原体存在的分子研究

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Aim: At present, routine laboratory investigation of the infectious agents implicated in female genital infections is mainly based on culture/direct fluorescence antibody (DFA) (immunofluorescence antibody test) results of cervicovaginal secretions. In this study the use of the menstrual tissue is introduced for the molecular detection of pathogens which are implicated in female infertility. Material and Methods: Cervicovaginal secretions and menstrual tissue samples of 87 women (mean age 34.07 ± 5.17) experiencing infertility problems were screened for Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis presence using polymerase chain reaction (PCR, light cycler-PCR). Cervicovaginal secretions were also tested by the culture/DFA technique. The results were compared using the binomial test. Results: In the overall study group, the prevalence of C. trachomatis was 25.3%, 18.3%, and 13.8%, the prevalence of U. urealyticum was 18.3%, 16.09% and 12.6% and the prevalence of M. hominis was 13.7%, 19.5% and 8.0% in the menstrual tissue, cervicovaginal secretions using PCR and cervicovaginal secretions culture/DFA, respectively. A statistically significant difference was revealed between the two methods for all three microbes and between menstrual tissue and cervicovaginal secretions PCR for chlamydia. Conclusions: The use of menstrual tissue along with the PCR method seems to be an effective and thus novel alternative for the investigation of the infectious agents lying in the genital tract. One of the main advantages of this technique compared to cervicovaginal secretions is that it is non-invasive and the sample can be collected at home, thus allowing the early detection and treatment of a condition that can otherwise lead to serious consequences, such as tubal obstruction, pelvic inflammatory disease, ectopic pregnancy, spontaneous abortions and unexplained infertility.
机译：目的：目前，常规的实验室检查涉及女性生殖器感染的传染原主要基于宫颈阴道分泌物的培养/直接荧光抗体（DFA）（免疫荧光抗体试验）结果。在这项研究中，月经组织被用于分子检测病原体，这些病原体与女性不育症有关。材料和方法：使用聚合酶链反应（PCR，光循环仪-PCR）筛选87例不育问题女性（平均年龄34.07±5.17）的宫颈阴道分泌物和月经组织样本中的沙眼衣原体，解脲脲原体和人支原体。还通过培养/ DFA技术测试了宫颈阴道分泌物。使用二项式检验比较结果。结果：在整个研究组中，沙眼衣原体的患病率为25.3％，18.3％和13.8％，解脲脲原体的患病率为18.3％，16.09％和12.6％，人支原体的患病率为13.7％。分别在月经组织中的19.5％和8.0％，使用PCR的宫颈阴道分泌物和宫颈阴道分泌物培养物/ DFA。两种方法对所有三种微生物以及衣原体的月经组织和宫颈阴道分泌物PCR均显示出统计学上的显着差异。结论：将月经组织与PCR方法一起使用似乎是一种有效的方法，因此是研究生殖道感染因子的新选择。与宫颈阴道分泌物相比，该技术的主要优势之一是它是非侵入性的，并且可以在家中采集样本，因此可以及早发现和治疗可能导致严重后果（例如输卵管阻塞）的疾病，盆腔炎，异位妊娠，自然流产和无法解释的不孕症。

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