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首页> 外文期刊>The Journal of Nutritional Biochemistry >Molecular signature of kappa-carrageenan mimics chondroitin-4-sulfate and dermatan sulfate and enables interaction with arylsulfatase B.
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Molecular signature of kappa-carrageenan mimics chondroitin-4-sulfate and dermatan sulfate and enables interaction with arylsulfatase B.

机译：κ-角叉菜胶的分子标记模拟了硫酸软骨素4和硫酸皮肤素，并使其能够与芳基硫酸酯酶B相互作用。

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摘要

The common food additive kappa-carrageenan ( kappa-CGN) is a sulfated polysaccharide that resembles chondroitin-4-sulfate (C4S) and dermatan sulfate (DS). All have a sulfate group on C4 of a glycoside (galactose for CGN and N-acetylgalactosamine for C4S), and the sulfate-bearing glycoside is linked in a beta-1,4-configuration to an unsulfated, six-carbon sugar (galactose for CGN, glucuronate for C4S and iduronate for DS). The enzyme arylsulfatase B (ARSB; N-acetylgalactosamine-4-sulfate) is the highly selective enzyme that removes the four-sulfate group from the nonreducing terminus of C4S and DS, thereby regulating subsequent degradation. In this report, kappa-CGN is shown to be a substrate for recombinant human ARSB (rhARSB). Sulfate was generated from both C4S and kappa-CGN following incubation with rhARSB. Exposure of human colonic epithelial cells to kappa-CGN, but not to C4S, produced reactive oxygen species (ROS) and increased interleukin (IL)-8 secretion. The ROS production from kappa-CGN was reduced by exposure to rhARSB, but increased by competition from C4S or DS, but not from chondroitin-6-sulfate. Prior treatment of either lambda- or iota-CGN with rhARSB had no impact on ROS, IL-8 or inorganic sulfate production, demonstrating a specific effect of the molecular configuration of kappa-CGN. By mimicry of C4S and DS and by interaction with ARSB, kappa-CGN can directly interfere with the normal cellular functions of C4S, DS and ARSB. Since C4S and DS are present in high concentration in tissues, the impact of kappa-CGN exposure may be due to some extent to interference with the normal biological functions of ARSB, C4S and DS
机译：常见的食品添加剂kappa-carrageenan（kappa-CGN）是一种硫酸化多糖，类似于4-硫酸软骨素（C4S）和硫酸皮肤素（DS）。均在糖苷的C4上具有硫酸盐基团（对于CGN为半乳糖，对于C4S为N-乙酰半乳糖胺），带有硫酸盐的糖苷以β-1,4-构型与未硫酸化的六碳糖（ CGN，对于C4S为葡萄糖醛酸，对于DS为葡萄糖醛酸。芳基硫酸酯酶B（ARSB; N-乙酰半乳糖胺-4-硫酸盐）是一种高度选择性的酶，可从C4S和DS的非还原末端除去四硫酸盐基团，从而调节后续降解。在该报告中，kappa-CGN被证明是重组人ARSB（rhARSB）的底物。与rhARSB孵育后，C4S和Kappa-CGN均生成硫酸盐。人类结肠上皮细胞暴露于kappa-CGN，而不暴露于C4S，会产生活性氧（ROS）和白介素（IL）-8分泌增加。暴露于rhARSB可以降低kappa-CGN产生的ROS，但可以通过C4S或DS的竞争来提高ROS的产量，但不能用硫酸软骨素6的竞争来提高。用rhARSB预先处理lambda-或iota-CGN不会影响ROS，IL-8或无机硫酸盐的产生，这证明了kappa-CGN分子构型的特定作用。通过模仿C4S和DS以及与ARSB相互作用，κ-CGN可以直接干扰C4S，DS和ARSB的正常细胞功能。由于C4S和DS以高浓度存在于组织中，因此Kappa-CGN暴露的影响可能在某种程度上是由于干扰了ARSB，C4S和DS的正常生物学功能

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