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首页> 外文期刊>The Journal of Nutritional Biochemistry >Gender affects liver desaturase expression in a rat model of n3 fatty acid repletion
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Gender affects liver desaturase expression in a rat model of n3 fatty acid repletion

机译：性别影响n3脂肪酸补充大鼠模型中肝脏去饱和酶的表达

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摘要

Dietary n3 polyunsaturated fatty acids (PUFA) are major components of cell membranes and have beneficial effects on human health. Docosahexaenoic acid (DHA; 22:6n3) is the most biologically important n3 PUFA and can be synthesized from its dietary essential precursor, l-linolenic acid (ALA; 18:3n3). Gender differences in the efficiency of DHA bioconversion have been reported, but underlying molecular mechanisms are unknown. We compared the capacity for DHA synthesis from ALA and the expression of related enzymes in the liver and cerebral cortex between male and female rats. Wistar rats, born with a low-DHA status, were supplied with a suboptimal amount of ALA from weaning to 8 weeks of age. Fatty acid composition was determined by gas chromatography, the mRNA expression of different genes involved in PUFA metabolism was determined by RT-PCR (low-density array) and the expression of proteins was determined by Western blot analysis. At 8 weeks, DHA content was higher (+20 to +40%) in each phospholipid class of female livers compared to male livers. The 4, 5 and 6 desaturation indexes were 1.2-3 times higher in females than in males. The mRNA expression of 5- and 6-desaturase genes was 3.8 and 2.5 times greater, respectively, and the 5-desaturase protein was higher in female livers (+50%). No gender difference was observed in the cerebral cortex. We conclude that female rats replete their DHA status more readily than males, probably due to a higher expression of liver desaturases. Our results support the hypothesis on hormonal regulation of PUFA metabolism, which should be taken into account for specific nutritional recommendations.
机译：膳食中的n3多不饱和脂肪酸（PUFA）是细胞膜的主要成分，对人体健康具有有益的作用。二十二碳六烯酸（DHA; 22：6n3）是生物学上最重要的n3 PUFA，可以从其饮食必需的前体1-亚麻酸（ALA; 18：3n3）合成。据报道，DHA生物转化效率存在性别差异，但潜在的分子机制尚不清楚。我们比较了雄性和雌性大鼠从ALA合成DHA的能力以及相关酶在肝脏和大脑皮层中的表达。从断奶至8周龄时，向出生时具有低DHA状态的Wistar大鼠提供次优的ALA。通过气相色谱法测定脂肪酸组成，通过RT-PCR（低密度阵列）测定参与PUFA代谢的不同基因的mRNA表达，并通过蛋白质印迹分析测定蛋白质的表达。与男性肝脏相比，在女性肝脏的每个磷脂类别中，在8周时，DHA含量较高（+20至+ 40％）。女性的4、5和6脱饱和指数比男性高1.2-3倍。 5-和6-去饱和酶基因的mRNA表达分别是雌性肝脏的3.8倍和2.5倍，并且5-去饱和酶蛋白在女性肝脏中更高（+ 50％）。在大脑皮层中未观察到性别差异。我们得出的结论是，雌性大鼠比雄性大鼠更容易补充其DHA状态，这可能是由于肝脏去饱和酶的较高表达所致。我们的结果支持关于PUFA代谢的激素调节的假说，对于具体的营养建议应予以考虑。

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