• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Nutritional Biochemistry >Different mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells.
【24h】

Different mechanisms of cis-9,trans-11- and trans-10,cis-12- conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells.

机译：顺式 -9，反式 -11-和反式 -10，顺式 -12-共轭亚油酸的不同机理酸影响3T3-L1细胞中的脂质代谢。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Conjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 micro mol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L1 cells. Proteomics identified significant up- or down-regulation of 52 proteins by either CLA isomer. Protein and gene expression of uncoupling protein (UCP) 1, UCP3, perilipin and peroxisome proliferator-activated receptor (PPAR) alpha increased whereas UCP2 reduced for both CLA isomers. And eight-day treatment of trans-10,cis-12 CLA, but not cis-9,trans-11 CLA, significantly up-regulated protein and mRNA levels of PKA (P<.05), CPT-1 and TNF- alpha (P<.01). Compared to protein expression, both isomers did not significantly influence the mRNA expression of HSL, ATGL, ACO and leptin. In conclusion, high-dose, long treatment of cis-9,trans-11 CLA did not promote apoptosis, fatty acid oxidation and lipolysis in adipocytes, but may induce an increase in energy expenditure. trans-10,cis-12 CLA exhibited greater influence on lipid metabolism, stimulated adipocyte energy expenditure, apoptosis and fatty acid oxidation, but its effect on lipolysis was not obvious.
机译：共轭亚油酸（CLA）已显示可减少各种实验动物体内的脂肪量。确定其对涉及能量消耗，细胞凋亡，脂肪酸氧化和脂解的酶的影响是很有价值的。我们研究了高剂量，长期处理CLA（75.4 micro mol / L，8天）对培养的3T3-L1细胞中这些酶的蛋白质和基因表达的异构体特异性影响。蛋白质组学确定了CLA异构体对52种蛋白质的显着上调或下调。两种CLA异构体的解偶联蛋白（UCP）1，UCP3，perilipin和过氧化物酶体增殖物激活受体（PPAR）α的蛋白和基因表达均增加，而UCP2降低。而 trans -10， cis -12 CLA而不是 cis -9， trans 的八天治疗-11 CLA显着上调了PKA（ P <。05），CPT-1和TNF-α（ P <。01）的蛋白质和mRNA水平。与蛋白质表达相比，两种异构体均未显着影响HSL，ATGL，ACO和瘦素的mRNA表达。总之，大剂量，长期治疗 cis -9， trans -11 CLA不会促进脂肪细胞的凋亡，脂肪酸氧化和脂解作用，但可能诱导脂肪细胞增加在能源支出上。 trans -10，顺式 -12 CLA对脂质代谢，刺激脂肪细胞能量消耗，细胞凋亡和脂肪酸氧化的影响更大，但对脂解作用不明显。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号