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首页> 外文期刊>Biomolecular engineering >Reactive oxygen species and DNA damage after ultrasound exposure.
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Reactive oxygen species and DNA damage after ultrasound exposure.

机译：超声暴露后，活性氧和DNA受损。

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摘要

The aim of this work was to detect the formation of hydrogen peroxide and hydroxyl radicals after ultrasound (US) exposure and test the hypothesis that reactive oxygen species induced by ultrasound can contribute to DNA damage. Formation of reactive oxygen species was observed in incubated medium after sonication with 1 MHz continuous ultrasound at the intensities of 0.61-2.44 W/cm2. Free radicals and hydrogen peroxide produced by ultrasound exposure of cells can lead to DNA damage. Comet assay was used to assess the effect of ultrasound on the level of nuclear DNA damage. The nucleated erythrocytes from fish were exposed in vitro to ultrasound at the same intensities and frequency. It was noticed that ultrasound in all used intensities induced DNA damage. The effect was not eliminated by the addition of catalase, which indicates that DNA damage was not caused by hydrogen peroxide only. The results showed that the DNA damage can be repair and this mechanism was the most effective after 30 and 60 min after sonication. Furthermore, the ultrasound-induced DNA damage in the presence of sonosensitizer (Zn- and AlCl-phthalocyanine) was studied. It was noticed that phthalocyaniens (Pcs) alone or with ultrasound did not induce significant changes in the level of DNA damage.
机译：这项工作的目的是检测超声（US）暴露后过氧化氢和羟​​基自由基的形成，并检验由超声诱导的活性氧可导致DNA损伤的假说。在1 MHz连续超声以0.61-2.44 W / cm2的强度进行超声处理后，在培养的培养基中观察到了活性氧的形成。超声波照射细胞产生的自由基和过氧化氢可导致DNA损伤。彗星试验用于评估超声对核DNA损伤水平的影响。鱼的有核红细胞在体外以相同的强度和频率暴露于超声。注意到在所有使用强度的超声中都会引起DNA损伤。过氧化氢酶的加入并没有消除这种作用，这表明DNA损伤并非仅由过氧化氢引起。结果表明，DNA损伤可以修复，这种机制在超声处理30和60分钟后最有效。此外，研究了在声敏剂（Zn和AlCl-酞菁）存在下超声引起的DNA损伤。值得注意的是，单独或超声检查酞菁（Pcs）不会引起DNA损伤水平的明显变化。

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