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首页> 外文期刊>The Journal of Nutritional Biochemistry >Alanyl-glutamine resolves lipopolysaccharide-induced lung injury in mice by modulating the polarization of regulatory T cells and T helper 17 cells.
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Alanyl-glutamine resolves lipopolysaccharide-induced lung injury in mice by modulating the polarization of regulatory T cells and T helper 17 cells.

机译：丙氨酰谷氨酰胺通过调节调节性T细胞和T辅助17细胞的极化来解决小鼠脂多糖诱导的肺损伤。

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摘要

Regulatory T cells (Tregs) and T helper (Th) 17 cells are two subsets of Th cells with opposing actions which participate in the process of resolving acute lung injury (ALI). Glutamine (Gln) is a nutrient commonly used in nutrition regimens due to its immunomodulatory effects. This study investigated whether alanyl-glutamine (Ala-Gln) administration modulated polarization of Th subsets in a model of lipopolysaccharide (LPS)-induced ALI during the resolution period. Intratracheal instillation of LPS in male C57BL/6J mice was used to induce ALI. On day 1 after LPS instillation, mice in the Gln group were given an intragastric gavage of 0.75 g Ala-Gln/kg daily, whereas the control (Con) group received identical amounts of sterilized distilled water. At 4, 7 and 10 days after a single dose of LPS, mice were killed by cardiac puncture. Bronchoalveolar lavage (BAL) fluid and lung tissues were collected for further analysis. Compared to the Con group, weight loss was less in the Gln group. Percentages of Tregs and interleukin-2 levels in BAL fluid increased, whereas Th17 cells were suppressed in the Gln group. Neutrophil clearance was promoted in the Gln group. Expressions of proinflammatory-related and fibrosis-related genes in lung tissues decreased in the Gln group. Histopathological findings also showed that interstitial inflammation was less severe in the Gln group. These results suggest that Ala-Gln administration after the onset of ALI can help resolve lung inflammation and injury by modulating the polarization of Tregs and Th17 cells
机译：调节性T细胞（Tregs）和辅助T（Th）17细胞是具有相反作用的Th细胞的两个子集，它们参与解决急性肺损伤（ALI）的过程。谷氨酰胺（Gln）由于其免疫调节作用，是营养疗法中常用的营养素。这项研究调查了在分解过程中，脂多糖（LPS）诱导的ALI模型中，丙氨酰-谷氨酰胺（Ala-Gln）施用是否调节Th亚型的极化。气管内LPS在雄性C57BL / 6J小鼠中的滴注被用来诱导ALI。 LPS滴注后的第1天，Gln组的小鼠每天灌胃0.75 g Ala-Gln / kg的胃内灌胃，而对照组（Con）组接受相同量的无菌蒸馏水。在单剂LPS后第4、7和10天，通过心脏穿刺杀死小鼠。收集支气管肺泡灌洗液（BAL）和肺组织进行进一步分析。与Con组相比，Gln组的体重减轻较少。 BAL液中Treg和白介素2的百分比增加，而Gln组中Th17细胞被抑制。 Gln组促进中性粒细胞清除。 Gln组肺组织中促炎相关和纤维化相关基因的表达下降。组织病理学结果还显示，Gln组的间质炎症较轻。这些结果表明，ALI发作后给予Ala-Gln可以通过调节Tregs和Th17细胞的极化来帮助解决肺部炎症和损伤

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