• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Nutritional Biochemistry >mRNA transcripts as molecular biomarkers in medicine and nutrition
【24h】

mRNA transcripts as molecular biomarkers in medicine and nutrition

机译：mRNA转录物在医学和营养学中作为分子生物标志物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In medicine, mRNA transcripts are being developed as molecular biomarkers for the diagnosis and treatment of a number of diseases. These biomarkers offer early and more accurate prediction and diagnosis of disease and disease progression, and ability to identify individuals at risk. Use of microarrays also offers opportunity to identify orthogonal (uncorrelated) biomarkers not known to be linked with conventional biomarkers. Investigators are increasingly using blood as a surrogate tissue for biopsy and analysis; total RNA isolated from whole blood is predominantly from erythroid cells, and whole blood mRNA shares more than 80% of the transcriptome with major tissues. Thus blood mRNA biomarkers for individualized disease prediction and diagnosis are an exciting area in medicine; mRNA biomarkers in nutrition have potential application that parallels these opportunities. Assessment of selenium (Se) status and requirements is one area where tissue mRNA levels have been used successfully. Selenoprotein-H and selenoprotein-W as well as glutathione peroxidase-1 (Gpx1) mRNAs are highly down-regulated in Se deficiency in rat liver, and the minimum dietary Se requirement is 0.06-0.07 mu g Se/g based on these biomarkers, similar to requirements determined using conventional biomarkers. Blood Gpx1 mRNA can also be used to determine Se requirements in rats, showing that blood mRNA has potential for assessment of nutrient status. Future research is needed to develop mRNA biomarker panels for all nutrients that will discriminate between deficient, marginal, adequate and supernutritional individuals and populations, and differentiate between individuals who will benefit vs. be adversely affected by nutrient supplementation
机译：在医学上，mRNA转录物正在被开发为用于诊断和治疗多种疾病的分子生物标记。这些生物标记物提供了对疾病和疾病进展的早期且更准确的预测和诊断，并能够识别有风险的个体。微阵列的使用还提供了机会来识别未知的与常规生物标记物相关的正交（不相关）生物标记物。研究人员越来越多地使用血液作为替代组织进行活检和分析。从全血中分离出的总RNA主要来自类红细胞，并且全血mRNA与主要组织共享超过80％的转录组。因此，用于个体疾病预测和诊断的血液mRNA生物标志物是医学上令人兴奋的领域。营养中的mRNA生物标志物具有与这些机会平行的潜在应用。硒（Se）状况和需求的评估是成功使用组织mRNA水平的领域之一。硒缺乏症在大鼠肝脏中硒蛋白H和硒蛋白W以及谷胱甘肽过氧化物酶1（Gpx1）mRNA的表达都大大下调，根据这些生物标记物，最低饮食中硒的需求量为0.06-0.07μg Se / g，类似于使用常规生物标记物确定的要求。血液Gpx1 mRNA也可用于确定大鼠的硒需求量，表明血液mRNA有潜力评估营养状况。需要进一步的研究来开发所有营养素的mRNA生物标志物检测方法，以区分营养不足，边际，充足和超营养的个体和人群，并区分受益于营养补充剂或受营养补充剂不利影响的个体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号