• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomolecular engineering >On the use of ultrasounds to quantify the longitudinal threshold force to detach osteoblastic cells from a conditioned glass substrate
【24h】

On the use of ultrasounds to quantify the longitudinal threshold force to detach osteoblastic cells from a conditioned glass substrate

机译：关于使用超声波量化纵向阈值力以将成骨细胞从条件化玻璃基板上分离

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell adhesion on a biomaterial is an important phase of the cell-material interactions and the quality of this phase governs the success of the biomaterial integration. Understanding of the phenomena of cell adhesion and in particular understanding of cell adhesion on biomaterials is of crucial importance for the development of new biomaterials with excellent biocompatibility. One of the physical quantitative indexes to evaluate the quality of cell-material adhesion is its strength. Determining the strength of adhesive bonds requires applying external forces to the cells. Thus, a few methods have been developed to evaluate the strength of cell-material adhesion (micropipette, microplates, microcantilever,...). These methods apply shear forces on adherent cells. The aim of our work is the development of a new ultrasonic characterization method of cellular adhesion on substrates. With our method, longitudinal acoustic waves are applied on cell culture to impose a longitudinal strain on cells. Only the cells subjected to a sufficient level of strain will be detached from the substrate. The idea is to correlate cell detachment rate to the longitudinal strain threshold supported by cells. From this result, we can deduce the critical force just sufficient to detach the cell. This global method can be adapted for different cell types and for different substrates. This method can provide an evaluation of the effect of functionalization on substrates. The technique is investigated for the 200 kHz ultrasound frequency. An insonificator adapted to the use of cell culture boxes was developed and calibrated. Tests were carried out on a glass substrate with or without biological conditioning. We used the MC3T3-E1 osteoblastic cell line. Our results to date provide the value of the necessary force to detach with reproducibility osteoblastic cells from glass. (C) 2007 Elsevier B.V. All rights reserved.
机译：细胞粘附在生物材料上是细胞与材料相互作用的重要阶段，该阶段的质量决定了生物材料整合的成功。对细胞粘附现象的理解，特别是对生物材料上细胞粘附的理解，对于开发具有优异生物相容性的新型生物材料至关重要。评估细胞材料粘附质量的物理定量指标之一是它的强度。确定粘合强度需要对细胞施加外力。因此，已经开发了几种方法来评估细胞材料粘附的强度（微量移液器，微量滴定板，微量悬臂等）。这些方法将剪切力施加到贴壁细胞上。我们工作的目的是开发一种新的超声表征细胞粘附在基材上的方法。使用我们的方法，将纵向声波应用于细胞培养，以在细胞上施加纵向应变。只有经受足够水平应变的细胞才会从底物上脱落。想法是使细胞脱离速率与细胞支持的纵向应变阈值相关。从这个结果，我们可以推断出足以分离细胞的临界力。这种全局方法可以适用于不同的细胞类型和不同的底物。该方法可以提供功能化对基材的作用的评估。对200 kHz超声频率研究了该技术。开发并校准了适合于细胞培养箱使用的声波发生器。在有或没有生物调节的玻璃基板上进行测试。我们使用了MC3T3-E1成骨细胞系。迄今为止，我们的结果提供了将可重现性成骨细胞从玻璃上分离所需的力的价值。 （C）2007 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号