Cost effective method for construction of high quality cDNA libraries.

Figueiredo A; Balde A; Cardoso H; Pais MS

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Cost effective method for construction of high quality cDNA libraries.

机译：经济高效的方法来构建高质量的cDNA文库。

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摘要

A PCR-based method for the synthesis of high quality cDNA is described. This method combines the reverse transcriptase template switching with the assisted recombination cloning. It enables 5' end enrichment, cDNA synthesis from limiting starting material, elimination of digestion and subcloning steps and substantial time and cost savings.

机译：描述了一种基于PCR的合成高质量cDNA的方法。该方法将逆转录酶模板切换与辅助重组克隆相结合。它可以实现5'末端富集，从有限的起始原料合成cDNA，消除消化和亚克隆步骤，并节省大量时间和成本。

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《Biomolecular engineering》 |2007年第4期|共3页
作者
Figueiredo A; Balde A; Cardoso H; Pais MS;
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正文语种 eng
中图分类遗传学;医用一般科学;
关键词
DNA; Complementary; Financial cost; g lt; 3gt; limitin; Materials; DNA; 互补;

机译：DNA;Complementary;Financial cost;g 3;limitin;Materials;DNA;互补;

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专利

1. Cost effective method for construction of high quality cDNA libraries. [J] . Figueiredo A, Balde A, Cardoso H, Biomolecular engineering . 2007,第4期

机译：经济高效的方法来构建高质量的cDNA文库。
2. A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology [J] . Morozumi Takeya, Toki Daisuke, Eguchi-Ogawa Tomoko, BioTechniques . 2011,第3期

机译：通过转座子插入和Gateway技术的结合，快速，经济高效地对合并的cDNA克隆进行测序
3. An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available [J] . Korpelainen Helena, Kostamo Kirsi Journal of Biotechnology . 2010,第1期

机译：一种改进且经济高效的cDNA-AFLP方法，在无法获得高通量测序时研究转录衍生产物
4. Nomenclature of Works and Costs for Ensuring the Quality of Construction Products.Identification Methods. [C] . Pavel Zhuravlev, Evgeniy Bachus, Irina Markova Annual Russian-Polish-Slovak Seminar Theoretical Foundation of Civil Engineering . 2020

机译：确保建筑产品质量的工程和成本的命名。
5. A methodology to identify and categorize costs of quality deviations in design and construction. [D] . Farrington, Jodi J. 1987

机译：在设计和施工中识别和分类质量偏差成本的方法。
6. Bacteriophage lambda cloning system for the construction of directional cDNA libraries. [O] . P S Meissner, W P Sisk, M L Berman 1987

机译：噬菌体λ克隆系统用于定向cDNA文库的构建。
7. Bacteriophage lambda cloning system for the construction of directional cDNA libraries. [O] . Meissner, P S, Sisk, W P, Berman, M L 1987

机译：噬菌体λ克隆系统用于定向cDNA文库的构建。

Cost effective method for construction of high quality cDNA libraries.

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