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首页> 外文期刊>Biomolecular engineering >Cellular behavior of neointima-like cells onto Vitamin E-enriched poly(D,L)lactic acid
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Cellular behavior of neointima-like cells onto Vitamin E-enriched poly(D,L)lactic acid

机译：新内膜样细胞在富含维生素E的聚（D，L）乳酸上的细胞行为

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In-stent restenosis is a process that occurs in 10-50% of cases currently treated with stent and it is caused by an abnormal smooth muscle cell (SMC) proliferation and migration in the vascular lumen. One of the most promising strategy to reduce restenosis is stent coating with biodegradable polymers to deliver in situ anti-proliferative drugs. Poly(D,L)lactic acid (P(D,L)LA), one of the most interesting candidate for stent coating, has been observed to induce inflammation and neointimal proliferation. In our laboratory, we developed P(D,L)LA enriched with Vitamin E (Vit.E), an anti-oxidative and anti-inflammatory agent that reduces also SMC proliferation. In order to evaluate the in vitro cellular behaviour of neointima cells onto Vitamin E-enriched P(D,L)LA, cell adhesion and proliferation along with the expression of two SMC migration markers (MMP-9 and hyaluronic acid receptor CD44) were measured in rat vascular SMC A 10 cells seeded onto control P(D,L)LA (PLA) and P(D,L)LA films containing 10-30% (w/w) Vit.E (PLA10-30). Cell adhesion, proliferation and MMP-9 production were unaffected by the Vit.E presence in the PLA films after 24 h, while proliferation was slowed or blocked after 48 and 72 h onto PLA10, 20 and 30. MMP-9 production was almost blocked and CD44 density decreased significantly after 72 h for cells grew onto PLA30 compare to cells seeded onto PLA. These data indicate that Vit.E-enriched P(D,L)LA could be an interesting polymer for stent coating. (C) 2007 Elsevier B.V. All rights reserved.
机译：支架内再狭窄是目前用支架治疗的病例的10％至5​​0％发生的过程，它是由异常的平滑肌细胞（SMC）增殖和血管腔内迁移引起的。减少再狭窄的最有前途的策略之一是用可生物降解的聚合物涂覆支架，以递送原位抗增殖药。聚（D，L）乳酸（P（D，L）LA），最感兴趣的支架涂层候选材料之一，已被发现可引起炎症和新内膜增生。在我们的实验室中，我们开发了富含维生素E（Vit.E）的P（D，L）LA，这是一种抗氧化和消炎剂，可减少SMC的增殖。为了评估新内膜细胞在富含维生素E的P（D，L）LA上的体外细胞行为，测量了细胞黏附和增殖以及两种SMC迁移标志物（MMP-9和透明质酸受体CD44）的表达。在大鼠血管SMC A中，将10个细胞接种到含有10-30％（w / w）Vit.E（PLA10-30）的对照P（D，L）LA（PLA）和P（D，L）LA膜上。 24小时后，PLA膜中Vit.E的存在不会影响细胞粘附，增殖和MMP-9的产生，而在48、72小时后，在PLA10、20和30上，增殖减慢或受阻。MMP-9的产生几乎被阻滞与接种到PLA上的细胞相比，细胞在PLA30上生长72 h后CD44密度显着降低。这些数据表明，富含Vit.E的P（D，L）LA可能是用于支架涂层的有趣聚合物。 （C）2007 Elsevier B.V.保留所有权利。

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