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首页> 外文期刊>Biomolecular engineering >Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: Characterisation and influence on osteoblast behaviour.
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Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: Characterisation and influence on osteoblast behaviour.

机译：包含固定蛋白聚糖（PG）作为涂层的不同胶原蛋白类型的原纤维：表征及其对成骨细胞行为的影响。

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摘要

Collagen, the main organic component of bone, is used as a coating on titanium implants and as a scaffold material in bone tissue engineering. Surface modifications of titanium which promote osteoblast adhesion, proliferation and synthesis of collagen by osteoblasts are desirable. One biomimetic approach is the coating of titanium with collagen in fibrillar form. Other organic components of bone may be bound to fibrils and exert additional effects. In this study, the collagen types I-III were compared regarding their ability to bind the proteoglycans decorin and biglycan, which are found in bone. More collagen was bound to collagen II fibrils than to those of types I and III. Therefore, titanium surfaces were coated with fibrils of collagen type II containing biglycan or decorin or neither to investigate the effect of the proteoglycans on human primary osteoblast behaviour. In addition, the growth factor TGF-beta1 was adsorbed onto surfaces coated with fibrils of collagen type II containing biglycan ordecorin or neither to investigate the influence of decorin and biglycan on the effect of TGF-beta1 on osteoblasts. Fibril-bound biglycan and decorin influence primary osteoblast behaviour by themselves. The presence of substrate-bound biglycan or decorin influences the effect of TGF-beta1. These results may be important when designing collagen-based coatings or scaffolds for tissue engineering, including those loaded with growth factors.
机译：胶原蛋白是骨骼的主要有机成分，被用作钛植入物的涂层和骨骼组织工程中的支架材料。期望钛表面改性以促进成骨细胞粘附，成骨细胞增殖和胶原合成。一种仿生方法是用原纤维形式的胶原蛋白包被钛。骨骼的其他有机成分可能会与原纤维结合并发挥其他作用。在这项研究中，比较了I-III型胶原蛋白结合蛋白聚糖得体和双糖聚糖的能力，这些蛋白聚糖在骨骼中发现。与I型和III型相比，与II型胶原蛋白原纤维结合的胶原蛋白更多。因此，钛表面涂有含有双糖链蛋白聚糖或decorin或两者都不存在的II型胶原原纤维，以研究蛋白聚糖对人原代成骨细胞行为的影响。此外，将生长因子TGF-β1吸附在涂有含有双糖链蛋白或十一碳六烯或两者均不含的II型胶原原纤维涂层的表面上，以研究核心蛋白聚糖和双糖链蛋白对TGF-β1对成骨细胞的影响。原纤维结合的双糖链蛋白聚糖和核心蛋白聚糖本身会影响原代成骨细胞的行为。底物结合双链蛋白聚糖或decorin的存在影响TGF-beta1的影响。当设计用于组织工程的胶原基涂层或支架时，这些结果可能很重要，包括那些带有生长因子的涂层或支架。

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