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首页> 外文期刊>Biomolecular engineering >Growth factors upregulate deposition and remodeling of ECM by endothelial cells cultured for tissue-engineering applications.
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Growth factors upregulate deposition and remodeling of ECM by endothelial cells cultured for tissue-engineering applications.

机译：生长因子通过培养用于组织工程应用的内皮细胞上调ECM的沉积和重塑。

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Appropriate matrix formation, turnover and remodeling in tissue-engineered small diameter vascular conduits are crucial for their long-term function. The interaction between cells and extra-cellular components is indispensable in determining cellular behavior in tissues and on biomaterials. The fibrin that contains fibronectin shows promise in most aspects as a tissue engineering scaffold, whereas, deposition of elastin and collagen by endothelial cells grown in the lumen of the construct is desirable to improve post implant retention, mechanical stability and vaso-responsiveness. So far there is no report on production of extra-cellular matrix (ECM) proteins, elastin and collagen by endothelial cells (EC) in in vitro culture conditions. In this study, we have used a biomimetic approach of providing multiple growth factors (GF) in the fibronectin (FN)-containing fibrin matrix to induce production of elastin and collagen by the endothelial cells for application in vascular tissue engineering. Depositionof elastin and collagens with matrix remodeling is demonstrated through qualitative analysis of the matrices that were recovered after growing cells on the initial fibrin-FN-GF matrix. Expressions of mRNA for both proteins were assessed by real time polymerase chain reaction (RT-PCR) to estimate the effects of multiple growth factor compositions. Marked deposition of elastin and collagen was evidenced by staining the recovered matrix after different culture intervals. Obviously, the biomimetic environment created by adding angiogenic and platelet growth factors in the fibrin-fibronectin-gelatin matrix can induce deposition of collagens and elastin by EC.
机译：组织工程设计的小直径血管导管中适当的基质形成，更新和重塑对其长期功能至关重要。在确定组织和生物材料中的细胞行为时，细胞与细胞外成分之间的相互作用是必不可少的。包含纤连蛋白的纤维蛋白在大多数方面显示出作为组织工程支架的前景，而通过在构建体腔中生长的内皮细胞沉积弹性蛋白和胶原蛋白对于改善植入后保留，机械稳定性和血管反应性是理想的。到目前为止，还没有关于在体外培养条件下内皮细胞（EC）产生细胞外基质（ECM）蛋白，弹性蛋白和胶原蛋白的报道。在这项研究中，我们采用了一种仿生方法，即在含有纤连蛋白（FN）的纤维蛋白基质中提供多种生长因子（GF），以诱导内皮细胞产生弹性蛋白和胶原蛋白，从而用于血管组织工程。通过对基质进行定性分析，对在原始纤维蛋白-FN-GF基质上生长细胞后回收的基质进行定性分析，证明了弹性蛋白和胶原蛋白具有基质重构的作用。通过实时聚合酶链反应（RT-PCR）评估这两种蛋白质的mRNA表达，以评估多种生长因子组合物的作用。通过在不同的培养间隔后对回收的基质进行染色，可以证明弹性蛋白和胶原蛋白的沉积明显。显然，通过在纤维蛋白-纤连蛋白-明胶基质中添加血管生成因子和血小板生长因子而创造的仿生环境可以诱导EC沉积胶原蛋白和弹性蛋白。

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