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首页> 外文期刊>The Journal of Prosthetic Dentistry >Effects of triclosan on the cytotoxicity and fungal growth on a soft denture liner.
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Effects of triclosan on the cytotoxicity and fungal growth on a soft denture liner.

机译：三氯生对软义齿衬里的细胞毒性和真菌生长的影响。

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STATEMENT OF PROBLEM: Contamination of removable prostheses with microorganisms, particularly Candida albicans, is a common clinical problem. Microban, a broad-spectrum antimicrobial containing triclosan, recently has been proposed to inhibit microbial growth. PURPOSE: This study aimed to determine whether the addition of Microban to PermaSoft denture liner prevents the growth of C albicans and affects the cytotoxicity of the PermaSoft material. MATERIAL AND METHODS: Experimental specimen disks (5 x 1 mm each) with and without incorporated Microban were fabricated aseptically (n = 6) against polyester film to produce a smooth surface. To assess the cytotoxic effect of Microban, the MTT assay was used. To determine the effect of Microban on the growth of C albicans, disks were placed in Transwell dishes, covered with Sabouraud's broth containing an ATCC strain of C albicans, and incubated at 37 degrees C for 24 hours. Wells containing fluorocarbon resin disks or broth alone served as controls. The disks were rinsed to remove unattached C albicans and then sonicated in sterile water to remove surface organisms. Serial dilutions of the water extracts were plated on Sabouraud's agar and returned to the incubator for 24 hours. Colonies were counted with a Brunswick Colony Counter. Growth of C albicans in the internal aspects of the specimens was determined in a manner as previously described, with the exception that the specimens were sonicated to remove surface organisms, minced, and sonicated once more before making serial dilutions. The results were compared with ANOVA and Tukey intervals (alpha=.05). RESULTS: The number of colonies formed ranged from 17 to 31 x 10(5) (mean = 23 +/- 4 x 10(5)) and 14 to 69 x 10(5) (mean = 32 +/- 20 x 10(5)) for the PermaSoft with and without Microban groups, respectively. There was no statistically significant difference between PermaSoft with and without Microban. CONCLUSION: The addition of Microban did not significantly alter the cytotoxicity of the PermaSoft denture lining material or reduce the adherence of viable C albicans to the surface of PermaSoft material after 24 hours.
机译：问题陈述：可拆卸假体被微生物尤其是白色念珠菌污染是常见的临床问题。最近，有人提出了含三氯生的广谱抗菌剂Microban来抑制微生物的生长。目的：本研究旨在确定在PermaSoft假牙衬里添加Microban是否能防止白色念珠菌的生长并影响PermaSoft材料的细胞毒性。材料与方法：在无菌条件下（n = 6）在聚酯薄膜上制作带有和不带有Microban的实验样品盘（每个5 x 1 mm），以产生光滑的表面。为了评估Microban的细胞毒性作用，使用了MTT测定法。为了确定Microban对白色念珠菌生长的影响，将圆盘放在Transwell盘中，盖上含有白色念珠菌ATCC菌株的Sabouraud肉汤，并在37°C下孵育24小时。仅包含碳氟化合物树脂圆盘或肉汤的孔用作对照。冲洗圆盘以去除未附着的白色念珠菌，然后在无菌水中超声处理以去除表面生物。将水提取物的系列稀释液铺在Sabouraud琼脂上，并返回培养箱24小时。用不伦瑞克殖民地计数器对殖民地进行计数。用前述方法确定样品内部白色念珠菌的生长，不同的是，在进行系列稀释之前，对样品进行超声处理以去除表面生物，切碎并再次超声处理。将结果与方差分析和Tukey区间（alpha = .05）进行比较。结果：形成的菌落数量范围为17至31 x 10（5）（平均值= 23 +/- 4 x 10（5））和14至69 x 10（5）（平均值= 32 +/- 20 x 10 （5））分别用于带有和不带有Microban组的PermaSoft。有和没有Microban的PermaSoft之间没有统计学上的显着差异。结论：添加Microban不会在24小时后显着改变PermaSoft义齿衬里材料的细胞毒性或减少存活的白色念珠菌对PermaSoft材料表面的粘附。

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