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首页> 外文期刊>The Journal of Prosthetic Dentistry >Investigation into the nature of dentin resin tags: a scanning electron microscopic morphological analysis of demineralized bonded dentin.
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Investigation into the nature of dentin resin tags: a scanning electron microscopic morphological analysis of demineralized bonded dentin.

机译：牙本质树脂标签的性质研究：脱矿结合的牙本质的扫描电子显微镜形态分析。

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STATEMENT OF PROBLEM: While the formation of the dentin/adhesive hybrid layer has been generally established, the infiltration and flow of the adhesive resin inside the acid treated dentinal tubules remains controversial. PURPOSE: The aim of the present study was to investigate and review the current interpretation of resin tags by means of scanning electron microscopic (SEM) observation. MATERIAL AND METHODS: Eight noncarious, human third molars were cut transversally and then longitudinally to obtain 8 middle-to-deep dentinal surfaces. The dentin was etched with 37% phosphoric acid (H 3 PO 4 ) gel for 10 seconds and then rinsed with water for 20 seconds. The dentin was kept moist by removing the excess water with a damp cotton pellet. The conditioned dentin was treated with a dentin bonding agent (Single Bond) and was light-polymerized for 20 seconds. A 0.2- to 0.5-mm layer of flowable composite (Tetric Flow) was then applied to the bonded dentin followed by 2 layers (2 mm each) of composite (Z 250).Each composite was light-polymerized for 40 seconds. Subsequently, the specimens were cut lengthwise into 2 halves and randomly divided into 4 groups (n=4), according to the surface preparation modality of the sectioned surface: Group EA: ethylenediamine tetraacetic acid, Group PA3: H 3 PO 4, Group PA120: H 3 PO 4 + NaOCl, and Group CA: HCl + NaOCl. Two additional teeth (Group N) were cut lengthwise into 2 halves and served as the control. The sectioned surfaces were treated with HCl and NaOCl. All specimens were processed for SEM observation. RESULTS: Specimens from Groups EA, PA3, PA120, CA, and N showed filamentous structures that were tens of microns long. Some filaments presented split-ends with hollow structures and very thin walls. Others made sharp hairpin turns indicating they were soft and compliant. CONCLUSIONS: Conventional SEM techniques, which are currently used to detect resin tags, actually identified filamentous organic structures, supposedly glycosaminoglycans, which were resistant to conventional specimen preparation techniques. The organic component showed a strong resemblance to the lamina limitans contained within the dentinal tubules. Over-reliance on SEM morphology has led to much confusion about the depth of penetration of resin tags.
机译：问题陈述：虽然已经普遍确定了牙本质/粘合剂杂化层的形成，但在经过酸处理的牙本质小管内粘合剂树脂的浸润和流动仍存在争议。目的：本研究的目的是通过扫描电子显微镜（SEM）的观察来调查和审查树脂标签的当前解释。材料与方法：先将八个非龋齿的人类第三磨牙横向切开，然后纵向切开，以得到8个中至深的牙本质表面。用37％磷酸（H 3 PO 4）凝胶蚀刻牙本质10秒，然后用水漂洗20秒。用湿棉球去除多余的水分，保持牙本质湿润。用牙本质结合剂（Single Bond）处理经调理的牙本质，并使其光聚合20秒。然后将0.2到0.5毫米的可流动复合材料层（Tetric Flow）施加到粘结的牙本质上，然后施加2层（每个2毫米）复合材料（Z 250）。将每种复合材料进行光聚合40秒。随后，根据切片表面的表面制备方式，将样品纵向切成两半，随机分为4组（n = 4）：EA组：乙二胺四乙酸，PA3组：H 3 PO 4，PA120组：H 3 PO 4 + NaOCl，以及组CA：HCl + NaOCl。将另外两颗牙齿（N组）纵向切成两半，作为对照组。切片的表面用HCl和NaOCl处理。所有样品均进行了SEM观察。结果：EA，PA3，PA120，CA和N组的标本显示出数十微米长的丝状结构。一些细丝呈现出具有空心结构和非常薄的壁的开口端。其他人则发夹发尖，表明它们柔软而柔顺。结论：目前用于检测树脂标签的常规SEM技术实际上识别出丝状有机结构，据称是糖胺聚糖，对常规样品制备技术具有抵抗力。有机成分显示出与牙本质小管中所含的层状板极相似。对SEM形态的过度依赖导致对树脂标签渗透深度的困惑。

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