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首页> 外文期刊>The Journal of Veterinary Medical Science >Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis.
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Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis.

机译：通过单酶扩增片段长度多态性和脉冲场凝胶电泳对日本禽多杀性巴氏杆菌菌株进行分子流行病学研究。

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摘要

Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SEAFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan.Digital Object Identifier http://dx.doi.org/10.1292/jvms.10-0181
机译：利用单酶扩增片段长度，对1976年至2007年间日本不同禽类宿主的36种多杀巴斯德氏菌临床分离株进行了分子流行病学分析，包括来自美国，台湾和印度尼西亚的5种参考菌株。多态性（SE-AFLP）与基于经典 Apa 的脉冲场凝胶电泳（PFGE）的比较。结果，SE-AFLP提供了21个配置文件，而PFGE提供了20个配置文件。辛普森的多样性指数分析表明，SEAFLP比PFGE具有更高的辨别力。由此得出结论，SE-AFLP比PFGE具有更高的辨别力，可以区分禽类P。日本的多杀科分离株。另外，遗传学特征表明日本本土宿主中存在体细胞血清型3菌株的进化。数字对象标识符http://dx.doi.org/10.1292/jvms.10-0181

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