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首页> 外文期刊>The Journal of Veterinary Medical Science >Isolation and molecular characterization of methicillin-resistant staphylococci from horses, personnel and environmental sites at an equine hospital in Turkey.
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Isolation and molecular characterization of methicillin-resistant staphylococci from horses, personnel and environmental sites at an equine hospital in Turkey.

机译：从土耳其马医院的马匹，人员和环境场所分离耐甲氧西林葡萄球菌并进行分子鉴定。

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摘要

The present study was carried out to assess the frequency of methicillin-resistant staphylococci (MRS) among racehorses (n=209) and veterinary personnel (n=13) as well as environmental surfaces (n=14) at an equine hospital in Adana, Turkey. In addition, species distribution, antimicrobial susceptibility, resistance genes, staphylococcal chromosomal cassette mec (SCCmec) type and clonality of these isolates were also investigated. MRS were identified by 16S rRNA sequencing, and typed by pulsed-field gel electrophoresis (PFGE). As a result, MRS was isolated in horses (48.3%), clinic staff (92.3%) and environmental samples (71.4%). Of the 123 MRS isolates, 118 isolates were identified as Staphylococcus lentus, and the remaining ones were found to be S. sciuri (n=3), S. intermedius (n=1) and S. fleuretti (n=1). All isolates were found to be susceptible against vancomycin, quinupristin-dalfopristin and rifampicin. Additionally, single or various combinations of resistance genes were detected among MRS isolates. SCCmec type II was identified in all isolates. Similar PFGE patterns were observed among MRS isolated from horses, humans, and environmental samples. Since MRS were concurrently isolated from horses and humans it is suggested that cross-transmission of MRS between horses and humans might occur. However, it cannot be ruled out that transmission is human to animal or animal to human.
机译：本研究旨在评估阿达纳州一家马医院的赛马（n = 209）和兽医人员（n = 13）以及环境表面（n = 14）之间耐甲氧西林葡萄球菌（MRS）的频率，火鸡。此外，还研究了这些分离株的种类分布，抗菌药敏性，耐药基因，葡萄球菌染色体盒mec（SCCmec）类型和克隆性。通过16S rRNA测序鉴定MRS，并通过脉冲场凝胶电泳（PFGE）进行分型。结果，在马（48.3％），诊所工作人员（92.3％）和环境样本（71.4％）中分离出MRS。在123个MRS分离株中，鉴定出118个分离株为慢链球菌，其余为S. sciuri（n = 3），inter。intermedius（n = 1）和S. fleuretti（n = 1）。发现所有分离株均对万古霉素，奎奴普丁-达福普汀和利福平敏感。另外，在MRS分离株中检测到抗性基因的单个或多种组合。在所有分离株中均鉴定出II型SCCmec。在从马，人和环境样品中分离出的MRS中，观察到类似的PFGE模式。由于MRS同时从马和人中分离出来，因此建议在马和人之间发生MRS的交叉传播。但是，不能排除传播是人与动物之间或动物与人之间的传播。

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