Development of a Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus equi subsp zooepidemicus and Analysis of Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory Tract Specimens

Kinoshita Yuta; Niwa Hidekazu; Katayama Yoshinari

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Development of a Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus equi subsp zooepidemicus and Analysis of Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory Tract Specimens

机译：循环介导的等温扩增方法的开发，用于检测马链球菌兽疫亚种，并与三种简单的从马呼吸道标本中提取DNA的方法进行分析

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Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a dominant pathogenic bacterium in equine pneumonia. We developed a specific loop-mediated isothermal amplification (LAMP) method, which targets the gene encoding sorbitol-6-phosphate 2-dehydrogenase (sorD), for detecting S. zooepidemicus and examined the clinical efficacies of its use in combination with each of 3 DNA extraction methods easily used by veterinary practitioners, namely the Loopamp PURE DNA Extraction Kit, InstaGene Matrix and a conventional boiling method. The LAMP method plus the Loopamp PURE DNA Extraction Kit gave higher rates of positivity than the other combinations in both clinical and spiked samples containing clinically significant concentrations (>1 x 10(4) CFU/ml) of S. zooepidemicus.

机译：马链球菌亚种。兽疫（S. zooepidemicus）是马肺炎的主要致病菌。我们开发了一种特异性的环介导的等温扩增（LAMP）方法，该方法的目标是编码山梨醇-6-磷酸酯2-脱氢酶（sorD）的基因，用于检测兽疫链球菌，并结合使用3种方法分别检查其临床疗效兽医从业人员容易使用的DNA提取方法，即Loopamp PURE DNA提取试剂盒，InstaGene Matrix和常规煮沸方法。 LAMP方法加上Loopamp PURE DNA提取试剂盒在含有临床显着浓度（> 1 x 10（4）CFU / ml）兽疫链球菌的临床样品和加标样品中的阳性率均高于其他组合。

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《The Journal of Veterinary Medical Science》 |2014年第9期|共5页
作者
Kinoshita Yuta; Niwa Hidekazu; Katayama Yoshinari;
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正文语种 eng
中图分类动物医学（兽医学）;
关键词
DNA extraction method; equine; LAMP method; Loopamp PURE DNA Extraction Kit; Streptococcus equi subsp zooepidemicus;

机译：DNA提取方法;马;LAMP方法;Loopamp PURE DNA提取试剂盒;马链球菌兽疫亚种;

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1. Development of a Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus equi subsp zooepidemicus and Analysis of Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory Tract Specimens [J] . Kinoshita Yuta, Niwa Hidekazu, Katayama Yoshinari The Journal of Veterinary Medical Science . 2014,第9期

机译：循环介导的等温扩增方法的开发，用于检测马链球菌兽疫亚种，并与三种简单的从马呼吸道标本中提取DNA的方法进行分析
2. Development of a Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus equi subsp. zooepidemicus and Analysis of Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory Tract Specimens [J] . Hidekazu NIWA, Yoshinari KATAYAMA, Yuta KINOSHITA The Journal of Veterinary Medical Science . 2014,第9期

机译：环介导的等温扩增方法检测链球菌马亚种的开发。动物流行病及其从马呼吸道标本中提取DNA的三种简单方法的用途分析
3. Development of a Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus equi subsp. zooepidemicus and Analysis of Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory Tract Specimens [J] . Hidekazu NIWA, Yoshinari KATAYAMA, Yuta KINOSHITA The Journal of Veterinary Medical Science . 2014,第9期

机译：环介导的等温扩增方法检测链球菌马亚种的开发。动物流行病及其从马呼吸道标本中提取DNA的三种简单方法的用途分析
4. Using Loop-mediated Isothermal DNA Amplification (LAMP) and Spectral Surface Plasmon Resonance (SPR) to Detect Methicillin-resistance S. aureus (MRSA) [C] . Yang K.L.A., Wu S.Y., Kwok H.C., Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on . 2012

机译：使用环介导的等温DNA扩增（LAMP）和光谱表面等离子体共振（SPR）检测耐甲氧西林的金黄色葡萄球菌（MRSA）
5. Development of Isolation and Detection Methods for the Analysis of DNA and Bacteria [D] . Varona, Marcelino. 2021

机译：DNA和细菌分析分离和检测方法的发展
6. Development of a Loop-Mediated Isothermal Amplification Method for DetectingStreptococcus equi subsp. zooepidemicus and Analysisof Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory TractSpecimens [O] . Yuta KINOSHITA, Hidekazu NIWA, Yoshinari KATAYAMA 2014

机译：回路介导的等温扩增检测方法的发展马链球菌亚种。兽疫和分析三种简单方法从马呼吸道中提取DNA的合成标本
7. Molecular characterization of virulence genes of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in equines [O] . R. Javed, A. K. Taku, Rakhi Gangil, 2016

机译：链球菌患者毒力基因的分子表征。 EquI和链球菌等亚铜。 Zooepidemicus在车辆中
8. Simple Mini-Method to Extract DNA Directly from Soil for Use with PolymeraseChain Reaction Amplification [R] . Porteous, L. A., Armstrong, J. L. 1993

机译：简单的小法直接从土壤中提取DNa用于聚合酶链反应扩增

Development of a Loop-Mediated Isothermal Amplification Method for Detecting Streptococcus equi subsp zooepidemicus and Analysis of Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory Tract Specimens

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