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首页> 外文期刊>The journal of sexual medicine >Neuroanatomical evidence for a role of central melanocortin-4 receptors and oxytocin in the efferent control of the rodent clitoris and vagina.
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Neuroanatomical evidence for a role of central melanocortin-4 receptors and oxytocin in the efferent control of the rodent clitoris and vagina.

机译：神经解剖学证据表明中枢黑皮质素4受体和催产素在啮齿动物的阴蒂和阴道的传出控制中的作用。

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INTRODUCTION: The clitoris and the vagina are the main peripheral anatomical structures involved in physiological changes related to sexual arousal and orgasm. Their efferent control and, more particularly, the neurochemical phenotype of these descending neuronal pathways remain largely uncharacterized. AIM: To examine if brain neurons involved in the efferent control of the clitoris and the vagina possess melanocortin-4 receptor (MC4-R) and/or contain oxytocin (OT). METHODS: Neurons involved in the efferent control of the vagina and clitoris were identified following visualization of pseudorabies virus (PRV) retrograde tracing. PRV was injected into the vagina and clitoris in adult rats in estrous. On the fifth day postinjection, animals were humanely sacrificed, and brains were removed and sectioned, and processed for PRV visualization. The neurochemical phenotype of PRV-positive neurons was identified using double or triple immunocytochemical labeling against PRV, MC4-R, and OT. Double and triple labeling were quantified using confocal laser scanning microscopy. MAIN OUTCOME MEASURE: Neuroanatomical brain distribution, number and percentage of double-labeled PRV/MC4-R and PRV-/OT-positive neurons, and triple PRV-/MC4-R-/OT-labeled neurons. RESULTS: The majority of PRV immunopositive neurons which also expressed immunoreactivity for MC4-R were located in the paraventricular and arcuate nuclei of the hypothalamus. The majority of PRV positive neurons which were immunoreactive (IR) for OT were located in the paraventricular nucleus (PVN), medial preoptic area (MPOA), and lateral hypothalamus. PRV positive neurons were more likely to be IR for MC4-R than for OT. Scattered triple-labeled PRV/MC4-R/OT neurons were detected in the MPOA and the PVN. CONCLUSION: These data strongly suggest that MC4-R and, to a less extent, OT are involved in the efferent neuronal control of the clitoris and vagina, and consequently facilitate our understanding of how the melanocortinergic pathway regulates female sexual function.
机译：简介：阴蒂和阴道是涉及与性唤起和性高潮有关的生理变化的主要外周解剖结构。它们传出的控制，尤其是这些递减的神经元途径的神经化学表型在很大程度上仍未表征。目的：检查参与阴蒂和阴道传出控制的大脑神经元是否具有黑皮质素4受体（MC4-R）和/或含有催产素（OT）。方法：通过可视化伪狂犬病病毒（PRV）逆行追踪，鉴定出参与阴道和阴蒂传出控制的神经元。 PRV被发情地注入成年大鼠的阴道和阴蒂。注射后第五天，人道处死动物，取出大脑并切成薄片，进行PRV可视化处理。 PRV阳性神经元的神经化学表型使用针对PRV，MC4-R和OT的双重或三重免疫细胞化学标记进行鉴定。使用共聚焦激光扫描显微镜定量双重和三次标记。主要观察指标：神经解剖学大脑分布，双标记PRV / MC4-R和PRV- / OT阳性神经元以及三重PRV- / MC4-R- / OT标记神经元的数量和百分比。结果：大多数还表达对MC4-R免疫反应的PRV免疫阳性神经元位于下丘脑室旁和弓形核中。对OT具有免疫反应性（IR）的大部分PRV阳性神经元位于室旁核（PVN），视前内侧区域（MPOA）和下丘脑外侧。 PR4阳性神经元MC4-R较IR更可能为IR。在MPOA和PVN中检测到散布的三重标记PRV / MC4-R / OT神经元。结论：这些数据强烈表明，MC4-R和OT在较小程度上参与了对阴蒂和阴道的神经元传出控制，因此有助于我们了解黑皮质素能途径如何调节女性性功能。

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