• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The journal of sexual medicine >Expression of aquaporin water channels in the vagina in premenopausal women.
【24h】

Expression of aquaporin water channels in the vagina in premenopausal women.

机译：绝经前妇女阴道中水通道蛋白水通道的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

INTRODUCTION: Aquaporins (AQPs) are membrane proteins that facilitate water movement across biological membranes. This study builds on a previous report on the distinct localization of AQPs in the rat vagina. AIM: The purposes of this study were to investigate the localization and expression of the AQPs in the vaginal tissue of premenopausal women. METHODS: Anterior vaginal tissue was collected during transvaginal uterine myomectomy or hysterectomy from 10 premenopausal women (mean age, 40 years) for Western blot and immunohistochemistry. MAIN OUTCOME MEASURES: The expression and cellular localization of AQP1-9 were determined in the human vagina by Western blot and immunohistochemistry. RESULTS: Immunolabeling showed that AQP1 was mainly expressed in the capillaries and venules of the vagina, AQP2 was expressed in the cytoplasm of the epithelium, AQP3 was mainly associated with the plasma membrane of the vaginal epithelium, and both AQP5 and AQP6 were expressed in the cytoplasm throughout all vaginal epithelium. Western blot analysis revealed bands at 28 kDa for AQP1, 2, 3, 5, and 6 proteins. However, AQP4, 7, 8, and 9 were not detected. CONCLUSIONS: The distinct localization of AQPs in the human vagina suggests that AQP1, 2, 3, 5, and 6 may play an important role in vaginal lubrication in women.
机译：简介：水通道蛋白（AQPs）是一种膜蛋白，可促进水在生物膜上的移动。这项研究建立在先前关于大鼠阴道AQPs独特定位的报告的基础上。目的：本研究的目的是调查绝经前妇女阴道组织中AQP的定位和表达。方法：从10名绝经前妇女（平均年龄40岁）经阴道子宫肌瘤切除术或子宫切除术中收集阴道前组织，进行Western blot和免疫组化分析。主要观察指标：Western blot和免疫组化法检测AQP1-9在人阴道中的表达和细胞定位。结果：免疫标记显示AQP1主要在阴道的毛细血管和小静脉中表达，AQP2在上皮的细胞质中表达，AQP3主要与阴道上皮的质膜相关，AQP5和AQP6均在阴道上皮中表达。细胞质遍布整个阴道上皮。蛋白质印迹分析显示AQP1、2、3、5和6蛋白的28 kDa条带。但是，未检测到AQP4、7、8和9。结论：AQP在人类阴道中的独特定位表明，AQP1、2、3、5和6可能在女性阴道润滑中起重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号