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首页> 外文期刊>The journal of sexual medicine >Three phases of corporal tracing elicited by electrical field stimulation on rabbit corpus cavernosum smooth muscle in penile perfusion model.
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Three phases of corporal tracing elicited by electrical field stimulation on rabbit corpus cavernosum smooth muscle in penile perfusion model.

机译：在阴茎灌注模型中，电场刺激在兔海绵体平滑肌上引起的体质追踪分为三个阶段。

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INTRODUCTION: Nitric oxide (NO) has been shown to mediate electrical field stimulation (EFS)-caused smooth muscle relaxation. It is known that the neural control of penile erection involves adrenergic, cholinergic, and non-adrenergic-non-cholinergic (NANC) neuro-effector systems; however, the effects of EFS on adrenergic and cholinergic nerves are not clear. AIMS: To elucidate EFS-induced signal transductions involved in adrenergic, cholinergic, and NANC neuro-effector systems by using an in vitro penile perfusion model. METHODS: EFS was performed on penile corpus cavernosum smooth muscle from male New Zealand White rabbits, which was pre-contracted with L-phenylephrine (10 microM). We investigated the penile tracing elicited by EFS on tissues pre-incubated with guanethidine (Guan, 50 microM), tetrodotoxin (TTX, 10 microM), Nomega nitro-L-arginine-methyl ester (L-NAME, 1 mM), atropine (50 microM), or eserine (10 microM). MAIN OUTCOME MEASURES: The time-to-peak of each phase, the percentage of relaxation, and the area under the curve (AUC). RESULTS: We discovered an extraordinary phenomenon: three distinct phases elicited by EFS. Phase I was abolished by L-NAME. Phase II was decreased by eserine and Guan, but increased by L-NAME. Phase III was abolished by atropine, but enhanced by eserine and Guan. TTX diminished all three phases. The time to reach the top of phase I was delayed by TTX. The time to attain the peak of phase II was shortened by L-NAME, but delayed by TTX and atropine. The time to reach the top of phase III was shortened by L-NAME, eserine, and Guan. AUC was significantly decreased by L-NAME and TTX. CONCLUSIONS: EFS stimulated adrenergic, cholinergic, and NANC neuro-effector systems simultaneously. Phase I was related to the NO pathway. Phase II was multiply affected by self-recovery properties, and adrenergic and cholinergic nerves. Phase III was related to cholinergic nerves. The corporal tracing elicited by EFS was the balanced result of multiple factors.
机译：简介：一氧化氮（NO）已显示出介导电场刺激（EFS）引起的平滑肌松弛的作用。众所周知，阴茎勃起的神经控制涉及肾上腺素能，胆碱能和非肾上腺非胆碱能（NANC）神经效应系统。然而，EFS对肾上腺素能和胆碱能神经的作用尚不清楚。目的：通过体外阴茎灌注模型阐明肾上腺素能，胆碱能和NANC神经效应系统中涉及EFS诱导的信号转导。方法：EFS是在雄性新西兰白兔的阴茎海绵体平滑肌上进行的，该肌肉预先与L-去氧肾上腺素（10 microM）签约。我们研究了由EFS在与胍乙啶（Guan，50 microM），河豚毒素（TTX，10 microM），Nomega硝基-L-精氨酸甲酯（L-NAME，1 mM），阿托品（ 50 microM）或色氨酸（10 microM）。主要观察指标：每个阶段的峰值时间，弛豫百分比和曲线下面积（AUC）。结果：我们发现了一种不寻常的现象：EFS引发了三个不同的阶段。第一阶段被L-NAME废除了。色氨酸和关减少了II期，但L-NAME增加了。第三阶段被阿托品废除，但被色氨酸和关加强。 TTX减少了所有三个阶段。 TTX延迟了到达第一阶段顶部的时间。 L-NAME缩短了达到II期峰的时间，但被TTX和阿托品延迟了。 L-NAME，eserine和Guan缩短了到达第三阶段顶部的时间。 L-NAME和TTX显着降低了AUC。结论：EFS同时刺激肾上腺素能，胆碱能和NANC神经效应系统。第一阶段与NO途径有关。 II期受到自我恢复特性以及肾上腺素能和胆碱能神经的多重影响。第三阶段与胆碱能神经有关。 EFS引发的体质追踪是多种因素的平衡结果。

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