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首页> 外文期刊>The Journal of Veterinary Medical Science >Prevalence of tick-borne Rickettsia and Ehrlichia in Ixodes persulcatus and Ixodes ovatus in Tokachi District, Eastern Hokkaido, Japan.
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Prevalence of tick-borne Rickettsia and Ehrlichia in Ixodes persulcatus and Ixodes ovatus in Tokachi District, Eastern Hokkaido, Japan.

机译：日本北海道东部十胜地区的per虫和卵虫中of传播的立克次体和埃里希氏菌的患病率。

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摘要

DNA from 111 ticks collected by flagging in Tokachi district, Eastern Hokkaido, Japan were examined for infection with Rickettsia and Ehrlichia, by PCR and sequencing methodology. For Rickettsia, analysis of the partial sequence of the citrate synthase gene was successfully performed on 11 DNA samples from I. persulcatus, and 7 of them showed 99.8% identical with Rickettsia helvetica while the other 4 showed 99.8% identical with 'Candidatus Rickettsia tarasevichiae'. For Ehrlichia, a partial sequence of the 16S rRNA gene detected from I. persulcatus was 100% identical with that from Ehrlichia muris, and another DNA sample from I. ovatus showed 99.8% identical with Ehrlichia species detected from I. ovatus. The results suggest that the pathogens detected here might be distributed in this area..
机译：通过PCR和测序方法，对日本北海道东部十胜地区检举111只s的DNA进行了立克次体和埃希氏菌感染的检测。就立克次体而言，成功地对波斯紫花s草的11个DNA样品进行了柠檬酸合酶基因的部分序列分析，其中7个样品与瑞士立克次氏菌具有99.8％的同一性，而其他4个样品与“立陶宛立克氏体立克次体”的99.8％相同。 。对于埃希氏菌，从百日咳艾美氏菌中检测到的16S rRNA基因的部分序列与穆里氏埃里希氏菌中的16S rRNA基因具有100％的同一性，而另一种来自卵形伊索氏菌的DNA样品与从卵虫中检出的埃希氏菌中的99.8％相同。结果表明，此处检测到的病原体可能分布在该区域。

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