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首页> 外文期刊>The journal of sexual medicine >Rho kinase-related proteins in human vaginal arteries: an immunohistochemical and functional study.
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Rho kinase-related proteins in human vaginal arteries: an immunohistochemical and functional study.

机译：人类阴道动脉中Rho激酶相关蛋白：一项免疫组织化学和功能研究。

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INTRODUCTION: The calcium-sensitizing Rho A/Rho kinase pathway has been suggested to play a role in the control of nongenital vascular smooth muscle. Rho-associated kinases (ROKs) cause calcium-independent modulation of smooth muscle contraction, and have been demonstrated in the bladder, prostate, and corpus cavernosum. Until now, it is not known whether ROKs and related proteins play a role in the control of vaginal blood flow. AIM: To investigate by means of functional studies and immunohistochemistry the significance of the Rho pathway in human vaginal arteries. METHODS: Vaginal tissue was obtained from five postmenopausal women. Specimens were processed for immunohistochemistry for ROK1, ROK2, RhoA, and RhoGDI. Segments of sub-epithelial vaginal arteries were mounted in a tissue bath. Effects of Y27632 on the concentration-response curves to phenylephrine (Phe) or Phe-precontracted preparations were investigated. MAIN OUTCOME MEASURE: The expression of Rho kinases ROK1, ROK2, and the Rho-associated protein RhoGDI in human vaginal arteries was investigated by means of immunohistochemistry. Tissue bath studies were conducted in order to characterize the effects of the ROK inhibitor Y27632 on isolated vaginal arteries. RESULTS: A meshwork of alpha-actin immunoreactive arterioles was located in the sub-epithelium of human vaginal specimens. Immunoreactivities for ROK1, ROK2, RhoA, and RhoGDI were expressed in the smooth musculature of these arteries. At 0.1 and 1 microM Y27632, the contraction to Phe (10 microM) was 99 +/- 17% and 28 +/- 12% that of 124 mM K(+) . In Phe-contracted preparations, Y27632 produced relaxant responses. CONCLUSIONS: The activation of alpha(1) -adrenoceptors contracts sub-epithelial human vaginal arteries via ROK-sensitive mechanisms. A role for these signals in the regulation of vaginal blood flow might be considered.
机译：简介：钙敏感的Rho A / Rho激酶途径被认为在控制非生殖器血管平滑肌中起作用。 Rho相关激酶（ROK）引起钙依赖性的平滑肌收缩调节，并已在膀胱，前列腺和海绵体中得到证实。到目前为止，还不清楚ROKs和相关蛋白是否在控制阴道血流中起作用。目的：通过功能研究和免疫组化研究Rho途径在人阴道动脉中的重要性。方法：从五名绝经后妇女中获取阴道组织。处理了标本以进行ROK1，ROK2，RhoA和RhoGDI的免疫组化。将上皮下阴道动脉段安装在组织浴中。研究了Y27632对苯肾上腺素（Phe）或Phe预收缩制剂的浓度-响应曲线的影响。主要观察指标：采用免疫组织化学方法研究了人阴道动脉中Rho激酶ROK1，ROK2和Rho相关蛋白RhoGDI的表达。进行组织浸浴研究以表征ROK抑制剂Y27632对孤立的阴道动脉的作用。结果：α-肌动蛋白免疫反应小动脉网位于人阴道标本的亚上皮细胞中。 ROK1，ROK2，RhoA和RhoGDI的免疫反应性在这些动脉的平滑肌组织中表达。在0.1和1 microM Y27632处，对Phe（10 microM）的收缩是124 mM K（+）的99 +/- 17％和28 +/- 12％。在苯丙氨酸收缩的制剂中，Y27632产生松弛反应。结论：α（1）肾上腺素受体的激活通过ROK敏感机制收缩上皮下人阴道动脉。可以考虑这些信号在调节阴道血流中的作用。

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