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首页> 外文期刊>The journal of sexual medicine >Cryopreserved Penile Tunica Albuginea for Allotransplantation: A Morphological and Ultrastructural Investigation
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Cryopreserved Penile Tunica Albuginea for Allotransplantation: A Morphological and Ultrastructural Investigation

机译：冷冻保存的阴茎皮白膜用于同种异体移植：形态和超微结构研究。

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Introduction. Peyronie's disease, a connective tissue disorder of penile tunica albuginea (TA) associated with penile deformity, curvature, pain, and erectile dysfunction, is best managed surgically, but suitable graft biomaterials are not available. Aim. To establish whether cryopreservation affects human TA in view of its use in allotransplants. Methods. The effects on TA samples of the two most widely used tissue cryopreservation methods were investigated using an ad hoc panel of histochemical, immunohistochemical, and ultrastructural tests. Apoptotic cells were evaluated using the terminal deoxynucleotidyl transferase method of end labeling (TUNEL) assay. Main Outcome Measures. Assessment of tissue integrity and arrangement of collagen and elastic fibers in thawed TA. Results. Both cryofixation methods provided TA tissue suitable for use as graft material. Significant ultrastructural changes, namely, a greater diameter of collagen fibrils, were detected in sections preserved in liquid nitrogen; nonetheless, such increase never exceeded the normal range. The comprehensive panel of assays used proved suitable to characterize the thawed tissue. Conclusion. Human TA is suitable for cryopreservation; freezing at -80°C provides better results than preservation in liquid nitrogen.
机译：介绍。佩罗尼氏病是一种与阴茎畸形，弯曲，疼痛和勃起功能障碍相关的阴茎白膜性结缔组织疾病（TA），最好通过手术治疗，但尚无合适的移植生物材料。目标。鉴于冷冻保存在同种异体移植中的用途，确定冷冻保存是否影响人TA。方法。使用组织化学，免疫组织化学和超微结构试验的特设小组研究了两种最广泛使用的组织冷冻保存方法对TA样品的影响。使用末端标记的末端脱氧核苷酸转移酶法（TUNEL）测定评估凋亡细胞。主要观察指标。评估融化TA中组织的完整性以及胶原蛋白和弹性纤维的排列。结果。两种冷冻固定方法都提供了适合用作移植物材料的TA组织。在液氮中保存的切片中检测到显着的超微结构变化，即胶原原纤维的直径更大。但是，这种增加从未超出正常范围。所使用的全面分析方法证明适合表征融化的组织。结论。人TA适用于冷冻保存;与在液氮中保存相比，在-80°C冷冻可获得更好的结果。

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