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首页> 外文期刊>The journal of sexual medicine >Activation of Gastrin-releasing Peptide Receptors in the Lumbosacral Spinal Cord is Required for Ejaculation in Male Rats
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Activation of Gastrin-releasing Peptide Receptors in the Lumbosacral Spinal Cord is Required for Ejaculation in Male Rats

机译：雄性大鼠射精需要激活os腰脊髓中胃泌素释放肽受体。

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Introduction. Ejaculation is a complex reflex mediated by a spinal ejaculation generator located in the lumbosacral spinal cord and consisting of a population of lumbar spinothalamic (LSt) neurons. LSt neurons and their intraspinal axonal projections contain several neuropeptides, including gastrin-releasing peptide (GRP). Aim. To test the hypothesis that GRP is critically involved in mediating ejaculation by acting in autonomic and motor areas of the lumbosacral spinal cord, utilizing a physiological paradigm to investigate ejaculatory reflexes in isolation of supraspinal inputs. Methods. Dual immunohistochemistry for GRP and galanin was performed to investigate co-expression of GRP in LSt cells of control male rats. Next, anesthetized, spinalized male rats received intrathecal infusions of either GRP antagonist RC-3095 (0, 10, or 20nmol/10μL) or GRP (0, 0.2, 0.5nmol/10μL). Ejaculatory reflexes were induced by electrical stimulation of the dorsal penile nerve (DPN) which reliably triggers rhythmic increases in seminal vesicle pressure (SVP) and contractions of the bulbocavernosus muscle (BCM), indicative of the emission and expulsion phases of ejaculation, respectively. Main Outcome Measures. GRP in LSt cells was expressed as percentages of co-expression. SVP and electromyographic recording (EMG) of BCM activity following drug treatment and DPN stimulation were recorded and analyzed for numbers of SVP increases, BCM events and bursts. Results. GRP was exclusively expressed in LSt cells and axons. Intrathecal infusion of RC-3095, but not saline, blocked SVP increases and BCM bursting induced by DPN stimulation. Intrathecal infusions of GRP, but not saline, triggered SVP increases and BCM bursting in 43-66% of animals and facilitated SVP increases and BCM bursting induced by subthreshold DPN stimulation in all animals. Conclusion. These data support a critical role for GRP for control of the emission and expulsion phases of ejaculation in male rats by acting in LSt target areas in the lumbosacral spinal cord.
机译：介绍。射精是一种复杂的反射，由位于腰and部脊髓中的一个脊柱射精发生器介导，由一群腰椎膜丘脑（LSt）神经元组成。 LSt神经元及其脊柱内轴突投影包含几种神经肽，包括胃泌素释放肽（GRP）。目标。为了检验这一假设，GRP通过在腰and脊髓的自主神经和运动区域中发挥作用来关键地介导射精，利用一种生理学范式来研究射精反射，以孤立于脊髓上输入。方法。进行了针对GRP和甘丙肽的双重免疫组化研究，以研究GRP在对照组雄性大鼠LSt细胞中的共表达。接下来，麻醉的脊椎雄性大鼠接受鞘内注射GRP拮抗剂RC-3095（0、10或20nmol /10μL）或GRP（0、0.2、0.5nmol /10μL）。阴茎背神经（DPN）的电刺激可诱发射精反射，该电刺激可靠地触发精囊压力（SVP）的节律性增长和球海绵体肌肉（BCM）的收缩，分别指示射精的发射阶段和排出阶段。主要观察指标。 LSt细胞中的GRP表示为共表达的百分比。记录药物治疗和DPN刺激后BCM活动的SVP和肌电图记录（EMG），并分析SVP增加次数，BCM事件和爆发。结果。 GRP仅在LSt细胞和轴突中表达。鞘内注射RC-3095，但不注射生理盐水，可阻止DVP刺激引起的SVP升高和BCM破裂。鞘内注射GRP而非生理盐水会在所有动物中引发SVP升高和43-66％的动物BCM破裂，并促进亚阈值DPN刺激引起的SVP升高和BCM破裂。结论。这些数据支持GRP通过作用于腰spin部脊髓的LSt目标区域来控制雄性大鼠射精的发射和排出阶段的关键作用。

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